摘要
用免疫组织化学方法结合图象分析技术对饥饿状态大鼠胰岛Ａ、Ｂ细胞中胰高血糖素（Ｇｌｕｃａｇｏｎ，Ｇｌｕ）和胰岛素（Ｉｎｓｕｌｉｎ，Ｉｎｓ）的免疫反应强度进行定量分析。结果表明：与正常对照相比，饥饿大鼠胰岛Ａ细胞中的Ｇｌｕ含量明显下降，Ｂ细胞中Ｉｎｓ含量明显升高。提示饥饿可导致Ｇｌｕ释放增加，Ｉｎｓ释放减少。与饥饿５天大鼠组相比较，饥饿５天后静脉注射葡萄糖组９０ｍｉｎ后胰岛内Ｇｌｕ含量明显升高，Ｉｎｓ含量无显著变化。提示：静脉注射葡萄糖可快速作用于胰岛Ａ细胞，减少Ｇｌｕ释放，但其对Ｂ细胞作用缓慢。从而为进一步阐明葡萄糖对胰岛Ａ、Ｂ细胞的不同作用机制提供形态学依据。
In this study,the glucagon and insulin immunoreactivities in the fasting rat pancreas were quantitated by image analysis.The results showed that the glucagon immunoreactivity significantly decreased in fasting rats and that of insulin increased,as compared with that in normal rats.The glucagon immunoreactivity significantly increased after intravenous injection of glucose but no marked difference was found in insulin.These results suggest that the A cells of pancreas may be directly stimulated by nutrients,and the B cells in a different way.
出处
《中国应用生理学杂志》
CAS
CSCD
1997年第4期359-360,共2页
Chinese Journal of Applied Physiology
关键词
胰岛
饥饿
胰岛素
胰高血糖素
starvation
glucagon
insulin
immunohistochemistry: image analysis
