摘要
对江苏省25个禽源性大肠杆菌O_1、O_2和O_(78)分离株的外膜蛋白型进行了测定。用改良的N-十二烷酰肌氨酸法提取其外膜蛋白,经SDS-PAGE电泳后,用考马斯亮蓝法进行染色。结果,8个O_1分离株,9个O_2分离株分属2个OMP型,其中的1个为二者所共有;而8个O_(78)分离株的OMP型也与该型相同。表明从江苏省分离到的禽源性大肠杆菌具有多样性的OMP型,而且这3个血清型的分离株中存在着共同的OMP型。
Omp patterns of twenty-five isolates of Escherichia coli serotype O1, O2 and O78 of chicken origin from Jiangsu area were determined. The outer membrane proteins of these isolates were isolated with the improved N-lauroylsarcosine method and analyzed by SDS-PAGE. Major outer membrane proteins bands contributed for OMP patterns were visualized with Coomassie brilliant blue stainning. Two OMP patterns were divided in eight of O1 isolates and nine O2 isolates respectivly. Among these OMP patterns, 1 OMP pattern was shared by these 2 different O serotypes isolates, and eight of O78 isolates also shared this OMP pattern. These results indicated that the OMP patterns of avian pathogenic Escherichia coli isolates of O1,O2 and O78 from this area were heterogeneous, and the same OMP patterns was presented in these 3 serotypes.
出处
《微生物学报》
CAS
CSCD
北大核心
1997年第6期463-468,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金(39500110)
江苏省教委自然科学基金资助项目部分研究工作
关键词
家畜病原细菌
大肠杆菌
外膜蛋白型
Escherichia coli, Outer membrane protein pattern, SDS-PAGE, Coomassie brilliant blue stainning