圈卷产色链霉菌lon基因的克隆及酶切分析

CLONING AND ENDONUCLEASE DIGESTION OF lon GENE FROM STREPTOMYCES ANSOCHROMOGENES

圈卷产色链霉菌是从我国东北土壤中筛选的一株Nkkomycin产生菌。在固体基本培养基上具有典型的链霉菌发育分化特征。经诱变得到不产孢子的白色突变株和不能形成气生菌丝的光秃型突变株。部分白色突变株和全部光秃型突变株在形态分化受阻的同时,也失去了产生Nikkomycin的能力。表明在圈卷产色链霉菌中,参与形态分化的基因与抗生素生物合成基因可能密切相关。

Streptomyces ansochromogenes is a nikkomycin producer which has typical life cycle of Streptomyces differntiation on glucose asparagine medium. The lon gene product is an ATP-dependent serine proteinase. The lon gene might have some connection with physiological differentiation and also with the morphological differentiation in S. coelicolor. The lon-like gene of S. ansochromogenes was identified by hybridization and the Ion-like gene in 4.6kb PstI fragment was cloned. The physical map of this fragment and the localization of the target gene were preliminarily studied.