人参皂甙Rg3联合小剂量化疗抑制小鼠S-180肉瘤血管生成的作用

Experimental Study on Anti-angiogenesis in Mice with Sarcoma S-180 by Ginsenoside Rg3 Combined with Low-dose of Cyclophosphamide

目的:观察人参皂甙Rg3联合持续小剂量环磷酰胺(CTX)对小鼠S-180肉瘤肿瘤血管生成的影响、抑瘤作用及毒副反应。方法:建立S-180肉瘤小鼠移植性肿瘤模型,60只小鼠随机分为4组,分别为人参皂甙Rg3组(5 mg/kg)、CTX组(5 mg/kg)、联合治疗组(人参皂甙Rg3和CTX的联合治疗)及模型对照组。治疗结束后测定肿瘤组织重量、肿瘤微血管密度(MVD)和血管内皮生长因子(VEGF)表达情况。结果:人参皂甙Rg3组、CTX组和联合治疗组小鼠移植瘤的瘤重较对照组降低(P<0.05),肿瘤抑制率分别为36.12%、49.43%、71.10%,MVD及VEGF的表达较对照组均下降(P<0.05),其中联合治疗组更明显(P<0.05)。联合治疗组小鼠毒副作用较轻,生存质量较好。结论:小剂量CTX与人参皂甙Rg3联合应用显示出明显的抗血管生成协同作用,抑瘤效果显著,且毒副反应小。

Objective To evaluate the anti - angiogenetic effect of the combination of ginsenoside Rg3 and lowdose cyclophosphamide（CTX） in mice with sarcoma S- 180, and to observe the anti - tumor effect, toxicity, adverse reaction of the tumor bearing mice. Methods BALB/c sarcoma S - 180 mice were taken as the model and randomly divid-ed into 4 groups, i, e, the low- dose CTX（LDCTX） group, the ginsenoside Rg3 （Rg3） group, the low-dose CTX combined withginsenoside Rg3（LDCTX ＋ Rg3） group, and the model group.Tumor weight of mice, tumor micmvascular density（MVD） and vascular endothelial growth factor（VEGF） gene expression were detemfined after the therapeutic course. Results In the LDCTX gYoup, Rg3 gYOup, LDCTX ＋ Rg3 gYoup, tumor grew comparatively slower than in the model group（ P 〈 0.05）, the tumor inhibitory effect was 36.12%, 49.43% and 71.10% respectively. Compared with the model group, MVD and VEGF expression were lower in other three groups, and the lowering action was more significant when the two drugs were used in combination （ P 〈 0.05）. The combined therapy of CTX and ginsenoside Rg3 resulted in better quality of mice life. Conclusion The combination of low - dose CTX and ginsenoside Rg3 has obvious synergetic action of anti - angiogenesis and shows significant tumor inhibitory effect, with less toxic and adverse reactions than the treatment of CTX alone.