摘要
以UV-A处理津田芜菁和赤丸芜菁块根24h后提取总RNA,再用RT-PCR方法分别克隆BrPAL1和BrPAL2基因的结果表明,BrPAL1和BrPAL2的开放读码框为2 169bp,编码722个氨基酸。氨基酸序列分析显示,BrPAL1和BrPAL2与甘蓝型油菜苯丙氨酸解氨酶(PAL)的同源性达99%,第61～559的肽段具有PAL结构域。BrPAL1和BrPAL2的核苷酸序列在9个位置上存在差异,而推导的氨基酸序列仅在3个位置上有差异。BrPAL1和BrPAL2基因有高度同源性。Northern杂交结果显示,UV-A可以诱导BrPAL1和BrPAL2表达,基因的表达量与处理时间呈相关。
The roots of ‘Tsuda＇ turnip and ‘Yurugi Akamaru＇ turnip （Brassica rapa） were irradiated with UV-A light for 24 h, then the total RNA was isolated and BrPAL1 and BrPAL2 genes were cloned by RT-PCR method. The BrPAL1 and BrPAL2 genes included an open reading frame （ORF） of 2169 bp and encoded a protein of 722 amino acid. Amino acid sequence analysis showed that BrPAL1 and BrPAL2 were 99% identity to PAL of Brassica napus, and the PAL domain was in the sequence from 61 to 559. The nucleotides of BrPAL1 and BrPAL2 genes had 9-bp differences, as well as 3 in deduced amino acid sequence. BrPAL1 and BrPAL2 genes had high identity. The Northern blotting results showed that the expression of BrPAL1 and BrPAL2 could be induced by irradiation of UV-A and the gene expression was correlated with light-exposure time.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2008年第3期485-490,共6页
Plant Physiology Communications
基金
国家自然科学基金(30700560)
国家自然科学基金重点项目(30730078)。
关键词
芜菁
花青素
苯丙氨酸解氨酶基因
基因克隆与表达
序列分析
turnip
anthocyanin
phenylalanine ammonialyase gene （PAL）
gene cloning and expression
sequence analysis
