摘要
目的:探讨血红素氧合酶-1(hemeoxyenase-1,HO-1)和Toll样受体2/4(Toll-likereceptor2/4,TLR2/4)在直肠癌中的表达及意义。方法:应用免疫组织化学法和RT-PCR检测30例直肠癌组织中HO-1蛋白以及HO-1mRNA、TLR2/4mRNA的表达。结果:直肠癌组织中HO-1蛋白表达(13/30)明显高于正常组织(3/30)(P<0.05),HO-1mRNA表达也明显高于正常组织(t=15.24,P<0.05);直肠癌组织中TLR2/4mRNA的表达高于正常组织(P<0.05),且HO-1mRNA与TLR2/4mRNA表达之间存在相关性(P<0.05)。结论:HO-1和TLR2/4在直肠癌组织中高表达且呈正相关,提示参与直肠癌的发生、发展。
Objective: To investigate the expression and significance of hemeoxygenase-1 (HO-1)and Toll-like receptor 2/4 (TLR2/4) in rectal carcinoma. Methods: The expression of HO-1 protein and HO-1 mRNA,TLR2/4 mRNA in 30 cases of rectal carcinoma was measured using immunohistochemical method and reverse transcriptase-polymerase chain reaction (RT-PCR). Results: The expression of HO-1 protein in rectal carcinoma (13/30)was significantly higher than those in normal tissue(3/30)(P 〈0.05).The expression of HO-1 mRNA was also significantly higher than those in normal tissue (t =15.24, P 〈0.05);The expression of TLR2/4 mRNA in rectal carcinomawas higher than those in normal tissue(P 〈0.05), and HO-1 mRNA expression was associated with TLR2/4 expression (P 〈0.05). Conclusion: The expression of HO-1 and TLR2/4 was higher in rectal carcinoma and had association with each other.
出处
《中国现代普通外科进展》
CAS
2008年第3期248-250,254,共4页
Chinese Journal of Current Advances in General Surgery