猪HSD11B1和MyoG基因多态性与妊娠期长短的关系

Relationship between Polymorphism of HSD11B1 and MyoG Gene and Gestation Length in Pigs

选择HSD11B1和MyoG2个基因作为影响妊娠期长短的候选基因。在7个中外猪群体中采用PCR-RFLP技术研究了HSD11B1和MyoG2个基因多态性与妊娠期长短的关系。HSD11B1基因的PCR-Bsh1236Ⅰ-RFLP分析结果表明:清平猪、新清平猪母系、大白猪、长白猪、杜洛克猪、中国瘦肉猪新品系DIV1和DIV2系猪群中A等位基因的频率分别为0.538、0.752、0.522、0.941、1.000、0.889和0.594;除DIV2外,HSD11B1不同基因型与妊娠期长短无显著关系(P>0.05)。MyoG基因的PCR-MspⅠ-RFLP分析结果表明:清平猪、新清平猪母系、大白猪、长白猪、杜洛克猪、中国瘦肉猪新品系DIV1和DIV2系猪群中M等位基因的频率分别为1.000、0.534、0.370、0.115、0.094、0.382和0.243;清平猪中只存在MM基因型,其他群体中3种基因型皆有分布;新清平猪母系MM比NN基因型母猪妊娠期要短,且在经产长白猪中差异极显著(P<0.01)。

11βhydroxysteroid dehydrogenase-1（HSD11B1） and myogenin（MyoG） were selected as candidate genes influencing on gestation length in pigs. The present study analyzed relationship between HSD11B1 and MyoG genes polymorphisms by PCR-RFLP and gestation length in 7 pig populations. Results showed that the frequencies of the A allele of HSD11B1 gene based on PCR-Bsh1236 Ⅰ -RFLP analysis were 0. 538, 0. 752, 0. 522, 0. 941, 1. 000, 0. 889 and 0. 594, respectively in Qingping, New Qingping line, Large White, Landrace, Duroc, DIV1 and DIV2 of New line of Chinese lean-type. Except for DIV2 line, there was no any significant differences between genotypes of HSD11B1 and gestation length （P〉0.05）. The frequencies of the M allele of MyoG gene based on PCR- Msp Ⅰ-RFLP analysis were 1.000, 0. 534, 0. 370, 0. 115, 0. 094, 0. 382 and 0. 243, respectively in Qingping, New Qingping line, Large White, Landrace, Duroc, DIV1 and DIV2 of New line of Chinese lean-type. The Qingping pig breeds only presented genotype MM, the genetic polymorphisms of the locus were detected in the other populations. The gestation length of genotype MM is less than that of genotype NN and the difference is significant in multiple parity Landrace（P〈0.01）.