异丙酚对全身高温所致大鼠海马神经元Caspase-3表达及超微结构改变的影响

Effects of propofol on whole body hyperthermia-induced Caspase-3 expression and ultra-structure changes of the hippocampus in rats

目的观察异丙酚对全身高温（whole body hypetthermia，WBH）导致的大鼠海马神经元超微结构及天冬氨酸特异性半胱氨酸蛋白酶-3（Caspase-3）表达的影响。方法300～350g的健康雄性Wister大鼠63只，随机分为3组，每组21只：空白对照组（A组）、WBH异丙酚麻醉组（B组）和WBH水合氯醛麻醉组（C组）。采用热辐射法建立大鼠WBH模型，6h后处死大鼠，其中每组20只大鼠用免疫组织化学法检测其海马神经元Caspase-3的表达并计算其灰度值，每组1只大鼠用电镜观察其海马CA1区神经元的超微结构。结果A组大鼠海马区神经元无Caspase-3表达，B、C两组大鼠海马区神经元有Caspase-3表达，B组较C组Caspase-3表达平均灰度值减小。B、C两组大鼠海马神经元超微结构发生改变、细胞器水肿，部分线粒体空化，内质网稀疏，突触前膜、后膜结构不完整，可见自噬体结构；B组大鼠的神经元超微结构损害较C组轻。结论异丙酚能减轻WBH引起的大鼠海马神经元Caspase-3的表达和超微结构的损害。

Objective To explore the effects of propofol on whole body hyperthemlia（WBH）-induced Caspase-3 expression and uhm-structure changes of the hippocampus in rats. Methods Sixty-three male Wister rats weighed 300-350 g were randomly divided into 3 groups（ n = 21 in each）： the control group（A）, the WBH anesthetized with Chloral Hydrate group（B）, and the WBH anesthetized with 150 mg/kg propofol group （C）. Twenty rats＇ brains in each group were removed for determination of Caspase-3 expression by using the SABC immuno-histochemical technique, and one rat＇s brain in each group was isolated for electron microscopic examination of hippocampal neurons. Results There was no expression of Caspase-3 in group A. The expression of Caspase-3 in group C was decreased compared with that in group B（P 〈0.01）. In groups B and C, expansion and vacuolization of mitochondria, swollen neurons and apoptotic bodies appeared, which suggested that WBH could induce neuron injury, and the neuron injury in group C was more severe than that in group B. Conclusion Propofol could decrease the expression of Caspase-3 and ultra-structure damage induced by WBH.