载脂蛋白E基因多态性与2型糖尿病合并脑梗死的关系

Relationship between apolipoprotein E gene polymorphism and type 2 diabetes mellitus with arteriosclerotic cerebral infarction

目的研究载脂蛋白E(ApoE)基因多态性与2型糖尿病(T2DM)血脂及其脑梗死并发症的关系。方法选择136例山东汉族T2DM患者为T2DM组,其中64例合并脑梗死(ACI)者为T2DM合并ACI组、72例未合并ACI者为T2DM未合并ACI组;60例健康对照者为对照组,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测其ApoE基因多态性,并探讨ApoE基因多态性与血脂异常和脑梗死并发症的关系。结果①T2DM组与健康对照组间基因型与等位基因分布频率均无统计学意义;②T2DM患者中总胆固醇、低密度脂蛋白水平在等位基因∈4组最高,在∈2组最低,差异具有统计学意义(P<0.01);③T2DM合并脑梗死患者中∈4组11例,与无脑梗死糖尿病患者中∈4组4例相比,差异具有统计学意义(χ2=4.672,P<0.05)。结论ApoE∈4等位基因与高脂血症有关,且∈4等位基因可能是T2DM合并脑梗死的危险因子。

Objective To explore the relationship between apolipoprotein E （APOE） gene polymorphism and dyslipidemia as well as arteriosclerotic cerebral infarction （ACI）in type 2 diabetic patients. Methods 136 patients with type 2 diabetes mellitus, 64 patients with ACI and 72 patients without ACI, and 60 healthy controls were determined for the APOE gene polymorphism by PCRRFLP. Results O Differences of distribution frequency in genotype and allelomorphic gene between type 2 diabetic patients and controls were insignificant. ②The TC and LDL-c levels in group ∈ 4 were the highest while the lowest were in group ∈ 2 （P 〈 0.01）. ③ 11 cases of the ∈4 allelle were found in the type 2 diabetic group with ACI and4 were found in the type 2 diabetic group without A CI （ X^2 = 4. 672, P 〈 0.05）. Conclusion The APOE ∈ 4 allele is related to hyperlipoidemia and the∈4 allele is possibly a risk factor of type 2 diabetes mellitus.