己酮可可碱对NOD小鼠胰腺、脾脏Fas和FasL表达的影响

Effect of pentoxifylline on Fas and FasL expressions in pancreas and spleen of NOD mice

环磷酰胺加速NOD小鼠发病，腹腔注射己酮可可碱（pentoxifylline，PTX）后监测糖尿病发病率，检测胰岛细胞凋亡、胰岛素表达以及胰腺、脾脏Fas和FasL mRNA的表达。结果提示PTX组糖尿病发病率下降（P〈0．05），胰岛细胞凋亡减少，而胰岛细胞胰岛素的表达增加，胰腺FasL mRNA的表达下降（P〈0．05），Fas有下降趋势但差异无统计学意义，脾脏Fas和FasL mRNA表达明显增强（P〈0．05或P〈0．01）。

NOD mice were treated with pentoxifylline （PTX） to investigate the incidence of cyclophosphamide-accelerated diabetes, the apoptosis and the insulin expression of β-cells and expressions of Fas or FasL mRNA in both pancreas and spleen. The results showed that incidence of diabetes in PTX group was significantly lower compared with control group （ P 〈 0.05 ）. The apoptosis of β-cells was decreased in group with higher insulin expression level in islet cells. The expression of FasL mRNA in pancreas of PTX group was lower than that of control group（P 〈0.05 ）, and there was no significant difference in Fas mRNA expression between two groups. Both Fas and FasL mRNA levels in spleen of PTX group were much higher than those of control group（ P 〈0.05 or P 〈0.01 ）.