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胃癌NET-1、Ki-67表达及意义 被引量:9

Overexpression of NET-1 and Ki-67 and their significance in gastric carcinomas
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摘要 目的研究胃癌组织中NET-1和Ki-67表达与其预后的关系。方法分别应用免疫组化EnVision两步法检测86例胃癌组织中NET-1和Ki-67的表达,对两指标均阳性病例行免疫组化双标双染方法,原位检测两指标的表达特点和比邻关系。所有病例随访60月以上。结果胃癌组织中NET-1和Ki-67高表达分别为56.98%和74.42%,两指标阳性表达呈正相关(P<0.01)。两指标高表达分别与癌分化呈负相关(均P<0.01);与癌临床分期呈正相关(均P<0.05);与患者生存率呈负相关(均P<0.05),其3年、5年生存率均低于相应的低表达组或阴性组(P<0.05,P<0.01);NET-1表达还与癌淋巴结转移呈正相关(P<0.01)。结论NET-1、Ki-67基因蛋白高表达反映胃癌细胞群体增殖活性,上调癌进展,预后较差。 Purpose To investigate the overexpression of NET-1 and Ki-67 and their prognostic significance in gastric carcinomas. Methods The overexpression of NET-1 and Ki-67 Protein was detected in paraffin-embeded sections of 86 cases of gastric carcinomas by immunohistochemical methods (EnVision). Immunohistochemical dual color staining procedure was used to demonstrate the dual positive cases with NET-1 and Ki-67 and to show the adjacent relationship between NET-1 and Ki-67 on one section. All cases were followed up over 60 months. Results The overexpression rates of NET-1 and Ki-67 in gastric carcinomas were 56.98% and 74.42%, respectively. There was significantly positive correlation between the dual markers (P 〈0. 05). The overexpression of NET-1 and Ki-67 was negatively correlated with carcinoma differentiation (P 〈 0. 01 ) and positively correlated with tumor clinical stages( P 〈0. 05 )but negatively correlated with tumor survival rates (P 〈 0. 05 ). The survival rates within 3 and 5 years were significantly shorter in the overexpression groups of NET-1 and Ki-67 than that in low expression groups and the negative groups(P 〈0. 05 ,P 〈0. 01 ), respectively. In addition, the overexpression of NET-1 was also positively correlated with lymph node status of tumor (P 〈0. 01 ). Conclusions The overexpression of NET- 1 and Ki-67 gene reflects the activity of cell clonal proliferation and significantly upregulates the carcinogenesis, which accelerates the development and progression of gastric carcinomas.
出处 《临床与实验病理学杂志》 CAS CSCD 北大核心 2008年第3期305-308,共4页 Chinese Journal of Clinical and Experimental Pathology
基金 江苏省教育厅高新技术资助项目(JHO2-118) 南通市社会发展项目(s1001)
关键词 胃肿瘤 NET-1 KI-67 预后 stomach neoplasmas NET-1 Ki-67 prognosis
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  • 1陈莉 李德春 朱远源.NET-1表达与肝癌临床病理因素相关性探讨.中华肿瘤杂志,2007,27(4):248-250.
  • 2Serru V, Dessen P, Boucheix C, et al. Seuqence and expression of seven new tetraspans [ J ]. Biochim Biophys Acta, 2000,1478 ( 1 ) : 159 - 163.
  • 3Xu J, Stolk J A, Zhang X, et al. Identification of differentiatly expressed gene in human prostatic cancer using substraetion and microarray[J]. J Cancer Res,2000,60(6) :1677 -1682.
  • 4Chen L, Li D C ,Zhu Y Y . The Expression of NET-1 gene in human tissues[J]. U S Chin Int J Bethune, 2004,2(6) :16 -20.
  • 5Wollscheid V, Kuhne-Heid R,Stein I,et al. Idenlification of a new proliferation-Associated protein NET-1/CA-8 characteristic for a subset of high-grade cervical intraepithelial neoplasia and cervical carcinoma[ J ]. Int J Cancer,2002,99:771 - 775.
  • 6Yomemura Y, Oeyarna S, Sugiyama K, et al. Growth fraction in gastric carcinoma detemined with monoclonal antibody Ki-67 [ J ]. Cancer, 1990,65 : 1130 - 1134.
  • 7Schluter C,Duchrow M,Wohlenberg C,et al. The cell proliferationassociated antigen of antibody Ki-67:a very large, ubiquitous nuclear protein with numerous repeated elements, representing a flew kind of cell cycle-maintaining proteins[J]. J Cell Bio, 1993,123 (3) :513 - 522.
  • 8何松,陈莉.常见肿瘤的WHO病理分型和TNM分期[M]//陈莉,程纯,主编.现代病理学临床研究的基本问题.北京:科学出版社,2007.176-177.
  • 9Chen L,Wang Z W,Zhen X,et al. Association of NET-1 gene expression with human hepatocellular carcinoma [ J ]. Int J Surg Pathol,2007,15 (4) : 346 - 353.
  • 10Gerdes J, Lemke H, Baxsch H,et al. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67[J]. J Immunol, 1984,133 : 1710 - 1715.

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