富血小板血浆富生长因子液对兔异体冻干跟腱移植早期微血管生成的影响

Angiogenic effect of platelet-released growth factors from platelet-rich plasma at early stage of transplantation of freeze-dried Achilles tendon in rabbits

目的观察富血小板血浆（PRP）、富生长因子液（PRGF）对兔异体冻干跟腱移植重建前交叉韧带（ACL）后早期微血管生成的影响。方法共选择14只健康成年新西兰白兔。12只兔切除双侧ACL，每只兔随机一侧植入经等量等渗盐水浸泡后的兔异体冻干跟腱（NS组），另一侧植入经PRGF浸泡后的兔异体冻干跟腱（PRGF组）。2只兔设为空白组，仅切开一侧膝关节。术后2，4，6周对移植物行HE和免疫组织化学染色检查，Weidner法定量检测移植物微血管密度（MVD）。结果术后2，4，6周，NS组MVD分别为2．52±0．45，3．41±0．44，2．57±0．51；PRGF组MVD分别为3．56±0．81，4．91±0．46，3．01±0．75，两组比较，差异有统计学意义（P〈0．05，0．01）PRGF组在微血管长入跟腱的时间及深度方面优于NS组。结论PRGF能促进异体冻干跟腱移植重建ACL后早期微血管生成。

Objective To observe angiogenic effect＇of platelet-released growth factors （PRGF） from platelet-rich plasma （PRP） on microvessel formation at early stage after anterior cruciate ligament （ACL） reconstruction by freeze-dried Achilles tendon. Methods The study involved 14 rabbits, of which 12 rabbits were used as experiment group and the other 2 as control group. In the experiment group, after two sides of rabbit ACL were removed, freeze-dried Achilles tendon treated by PRGF was transplanted into random one side of the knee to substitute the original ACL （ PRGF group）, while the other side was transplanted with freeze-dried Achilles tendon treated only by normal saline （NS group）. Only one side of the knee was removed in the control group. The grafts were observed by HE and immunohistochemical staining 2, 4 and 6 weeks after operation. Microvessel density （MVD） was measured by Weidner method. Results Compared with NS group, MVD in PRGF group was significant higher at 2,4 and 6 weeks after operation （P 〈0.05）. MVD of NS group at 2,4 and 6 weeks after operation was 2.52 ± 0. 45, 3.41 ± 0.44 and 2.57 ± 0.51 respectively, but that of PRGF group at 2,4 and 6 weeks after operation was 3.56 ± 0.81,4.91 ± 0.46 and 3.01 ± 0.75 respectively （ P 〈 0.05 ）. The time of neovascular formation and the depth of vascular penetration into the grafts of the PRGF group were superior to those of NS group. Conclusion PRGF can significantly promote microvessel formation at early stage after ACL reconstruction with freeze-dried Achilles tendon.