连接蛋白37，40，43在失血性休克大鼠肠系膜上动脉中的表达变化及其意义

Expression changes of Cx37, Cx40 and Cx43 of superior mesenteric arteries and their significance following hemorrhagic shock in rats

目的连接蛋白（Cx）37，40，43在失血性休克大鼠肠系膜上动脉（SMA）中的表达变化及其意义。方法取休克后不同时相点的SD大鼠SMA，运用RT—PCR和Western blot检测Cx37，40，43的表达变化；运用反义寡脱氧核苷酸（AODN）转染技术检测Cx40，43对SMA反应性的影响。结果Cx37，40在休克后表达逐渐下降，休克后2h表达最低，休克后3，4h表达逐渐升高；Cx43在休克后即刻表达迅速降低，以后进行性表达升高，在休克后2h达最高点，休克后3，4h表达再次降低；Cx40 AODN可以增加血管环内膜依赖性的收缩和舒张反应；Cx43 AODN可以抑制血管环的收缩和舒张反应。结论Cx40和Cx43参与了失血性休克后内膜依赖的血管舒缩反应性的调节。Cx40具有抑制休克后内膜依赖的血管舒缩反应性的作用；Cx43具有促进休克后内膜依赖的血管舒缩反应性的作用。

Objective To observe the expression changes of Cx 37, Cx 40 and Cx 43 of superior mesenteric arteries （SMA） and their significance following hemorrhagic shock in rats. Methods SMA was removed from normal or shocked SD rats at different time points （ immediately, 30 minutes, 1, 2, 3 and 4 hours after shock） for detection of mRNA and protein expressions of Cx 37, Cx 40 and Cx 43. Furthermore, the effect of Cx 40 or Cx 43 （ Cx 40 AODN or Cx 43 AODN ） on SMA＇ s vasomotor reactivity induced by myricetin or acetylcholine was studied by antisense oligodeoxynucleotide. Results The expressions of Cx 37 and Cx 40 of SMA were gradually decreased within 2 hours after shock and significantly increased at 3 and 4 hours after shock. While expression of Cx 43 was gradually increased at 0.5, 1 and 2 hours after shock and significantly decreased at 3 and 4 hours after shock. Cx 40 AODN significantly increased the vasomotor reactivity induced by myricetin or acetylcholine while Cx 43 AODN exerted an inhibitive effect. Conclusions Gx 40 and Cx 43 participate in regulating endothelium-dependent vasomotor reactivity following hemorrhagic shock. Cx 40 has an inhibitive effects on endothelium-dependent vasomotor reactivity but Cx 43 can promote such reactivity.