NSE、S100及GFAP在视网膜母细胞瘤中的表达及意义

Expression of NSE,S100 and GFAP in retinoblastoma and its clinical significance

目的探讨神经元特异性烯醇化酶(NSE)、酸性钙结合蛋白(S100)及胶质纤维酸性蛋白(GFAP)在视网膜母细胞瘤(Rb)中的表达及临床意义。方法对32例Rb眼球摘除标本进行病理切片,观察其形态及免疫组化染色NSE、S100、GFAP表达,并分析与肿瘤分化程度及临床分期的关系。结果Rb临床分期与病理分期并不完全一致。免疫组化显示,未分化型Rb中NSE染色阳性,GFAP和S100均呈阴性;而高分化型肿瘤中NSE染色阳性,胶质细胞、瘤间神经纤维GFAP和S100染色呈阳性。结论钙化是肿瘤的特征表现,肿瘤坏死与瘤细胞分化相关。肿瘤含有NSE阳性的神经元性瘤细胞,高分化型肿瘤可见S100、GFAP阳性的胶质细胞。

Objective To explore the expression of neuron specific enolaes （NSE）, S protein（S100） and glial fibrillary acidic protein（GFAP） in retinoblastoma（Rb） and its clinical significance. Method The NSE, S100 and GFAP levels were determined in 32 cases of eyeball excision by immunohistochemistry, and the relation between their expressions and tumor differentiation and clinical stage were analyzed. Results The Rb pathological stage was not identical to the clinical stage. The immunohistochemistry results showed that NSE was positive in both differentiated and undifferentiated Rb, S100 and GFAP were negative in undifferentiated Rb, but S100 and GFAP were positive in nerve fibers and nerve glial cells among the high differentiated Rb. Conclusions Calcification is one of the features of Rb. Tumor necrosis is associated with its differentiation degree. There are positive NSE neuron cells in Rb, and S100 and GFAP double positive nerve glial cells in the high differentiated Rb.