摘要
目的:建立高效液相色谱法测定醋酸氯己定栓的含量。方法:以依利特Hypersil BDS-C18(5μm,4.6mm×250mm)为分析柱;0.02mol/L磷酸二氢钾(含0.3%三乙胺,用磷酸调pH至2.5)-乙晴(70:30)为流动相;流速为1.0ml/min;检测波长为259nm;柱温为室温;用外标法对其进行定量。结果:醋酸氯己定的浓度在10～70μg/ml范围内与峰面积呈良好线性关系,回归方程为:A=53.8923×C-15.3636,r=1.000,平均回收率为99.72%,RSD为1.11%(n=6)。结论:本方法操作简便、结果准确、重现性好,可用于醋酸氯己定栓的含量测定。
Objective：A HPLC menthod was established for the determination of Chlorhexidine Acetate Suppositories. Methods：Yilite Hypersil BDS-C18（5μm,4.6mm）〈 250mm） column was used. The mobile phase was the mixture of 0. 02mol/L Potassium Dihydrogen Phosphat （Conten 0. 3% Trirthylamine, adjusted to pH2.5 with Phosphoric Acid）- Acetonitrile（70：30） ; The flow rate was 1.0mL ·min^-1; The detection wavelength was 259nm. and the temperature of the column was the ambient temperature. The standard curve method was used for the quantitative detution of Isoprenaline hydrochloride. Results ：The standard curve for Isoprenaline hydrochloride was linear in the range of 10μg/ml -70μg/ml. Regression equation： A=53. 8923C-15. 3636,r=1. 000. The recoveries was 99. 72% ,RSD（%）was 1.11 （n= 6）. Conclusion：The method is easy, accurate and reproducible. It can be used for the determination of Isopre- naline hydrochloride injection.
出处
《华夏医学》
CAS
2008年第2期219-221,共3页
Acta Medicinae Sinica
关键词
醋酸氯己定栓
HPLC
含量测定
Chlorhexidine Acetate Suppositories
HPLC
determination
