燕麦肽提取工艺的初步研究

Study on extraction technology of oat peptide

以燕麦麸皮为原料,研究了燕麦肽的分离方法和条件;通过Alcalase和Flavourzyme的共同作用表明,燕麦肽利用离心方法分离比较好,条件为4000r/min,10min;在水解过程中加入Flavourzyme可以使燕麦肽含量提高一倍,水解度也从10.48%提高到16.50%,同时燕麦肽的苦味也能得到很大的改善。通过对其分子量的测定可知,Flavourzyme能使燕麦肽水解成游离的氨基酸。

Oat bran,as raw material,the separation method and conditions for oat peptide was investigated. The protein in the bran was hydrolyzed to peptide by the cooperation of alcalase enzyme and flavourzyme. The result showed that centrifugation was better for oat peptide separation in the condition of 4000r/min, 10min and appending flavourzyme in hydrolyzing could double the content of peptide and increase DH from 10.48% to 16.50%. And flavourzyme can make the hydrolyzation more downrightly. And the bitterness of oat peptide was improved significantly. From the determination to molecular weight of oat peptide,flavourzyme could hydrolyze it to dissociative amino acid.