粪链球菌转化合成L-瓜氨酸的研究

Study on the conversion of L-citrulline by Streptococcus faecalis

运用粪链球菌中精氨酸脱亚胺基酶转化L-精氨酸制备L-瓜氨酸。考察了pH、温度、转速、表面活性剂等多种因素对L-瓜氨酸产量的影响。最佳条件如下:反应体系0.4mol/L醋酸缓冲液,起始pH6.0,转化温度35℃,转速120r/min,CTAB浓度0.3g/L,0.4g菌体,反应时间30h,精氨酸浓度96g/L。

L-srginine can be converted to L-citrulline with arginine deiminase which was found in the Streptococcus faecalis. Many factors affecting on the production of L-citrulline were studied. The optimum conditions were as follows： 0.4mol/L acetate buffer, pH6.0, 35℃, 120r/min, 9 （CTAB） = 0.3g/L, 0.4g organisms, 30h,96g/L L-Arginine.