狂犬病毒的增殖及保藏效果的初步研究

A Primary Study on Multiplication and Preservation of Rabies Virus

狂犬病是人和家畜的一种急性中枢神经系统的疾病,严重地威胁人类和家畜的生命.狂犬病街毒的收集、增殖及对这些毒株进行妥当的保藏,有利于人们充分保护这些病毒资源,并为今后利用该病毒开展治病防病工作提供实验材料.将狂犬病街毒和北京株固定毒适应株(aG)分别接种豚鼠,收集有典型狂犬病症状并死亡的豚鼠脑毒,在-30℃低温冰箱中保藏9个月.实验结果表明,对照组(aG)经保藏后病毒存活率达100%,而7株狂犬病街毒病毒存活率仅达70%左右,因此对狂犬病街毒的增殖和保藏方法有待于今后进一步研究.

The guinea pigs were infected with the street rabies virus strain and adaptive Bei-jing fixed rabies virus strain (aG). The virus were collected from brain of dead guinea pig with typical symptom, and preserved at -30癈 for nine months. The results showed the sur-viving rate of control(aG) was 100%, the surviving rate of seven street rabies virus strains were 70% or so. Therefore, the methods should be studied further for multiplication and preservation of the street rabies virus.