中药鬼箭羽的化学成分研究Ⅱ

Studies on chemical constituents from Euonymus alatus Ⅱ

目的:研究鬼箭羽Euonymus alatus的化学成分及抗肿瘤活性。方法:利用柱色谱分离,经理化常数测定,结合~1H-NMR,^(13)C-NMR,EI-MS鉴定结构,利用台盼蓝法测试部分化合物的体外癌细胞生长抑制活性。结果:从鬼箭羽的95%乙醇提取物的石油醚、醋酸乙酯萃取层中共分离得到10个化合物,分别鉴定为(+)-松萝酸(1),苯甲酸(2),2-羟基-4-甲氧基-3,6-二甲基苯甲酸(3),羽扇豆醇(4),3β-羟基-30-降羽扇豆烷-20-酮(5),(2R,3R)-3,5,7,4′-四羟基二氢黄酮(6),山柰酚(7),5,7,4′-三羟基二氢黄酮(8),槲皮素(9),胡萝卜苷(10)。活性研究表明,化合物1,4,5对体外人类急性髓细胞性白血病细胞株HL-60的IG_(50)分别为27.78,32.22,26.19μmol。结论:化合物1,3,5,8为首次从该属植物中分离得到。化合物1,4,5对体外人类急性髓细胞性白血病细胞株HL-60具有一定的生长抑制活性。

Objective： To study the chemical constituents and antitumor activities of Euonymus alatus. Method： Compounds were isolated and purified repeatedly by silica gel, Sephadex LH -20 column chromatography, and their chemical structures were elucidated by their physicochemical properties and spectral data, such as ^1H-NMR, ^13 C-NMR and EI-MS. The trypan blue experiments in vitro were used to observe the antitumor activity of several compounds. Result： Ten compounds were obtained and identified as （ ＋ ）- usnic acid （1）, henzoic acid （2）, 2-hydroxy-4-methoxy-3, 6-dimethyl benzoic acid （3）, lupeol （4）, 3β-hydroxy-30-norlupan-20- one （5）, （2R, 3R）-3,5, 7, 4＇-tetrahydroxy flavanone （6）, kaempferol （7）, 5, 7, 4＇-trihydroxy flavanone （8）, quercetin （9） and daucosterol （10）. Conclusion： Compounds 1, 2, 5 and 8 were isolated from this genus for the first time. The trypan blue experiments in vitro showed that compounds 1, 4 and 5 had growth inhibitory effects on HL-60 cells.