混合稀土“常乐”对大鼠肾脏组织的氧化损伤

Effect of Changle (Rare Earth Complex) on Kidney Antioxidation in Rats

目的研究长期口服低剂量混合稀土"常乐"对大鼠肾脏自由基防御机能的影响。方法将清洁级Wistar大鼠随机分为对照组和5个不同剂量的混合稀土"常乐"组(0.1、0.2、2.0、10.0、20.0mg/kg),每组30只,雌、雄各半。混合稀土"常乐"组每日以灌胃方式给予上述不同剂量的混合稀土"常乐",对照组给予等量0.9%生理盐水。持续灌胃6个月后,检测肾组织中Cu-Zn超氧化物歧化酶(Cu-ZnSOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力和脂质过氧化物(LPO)含量。结果与对照组比较,雄性大鼠20.0、10.0mg/kg组和雌性大鼠20.0mg/kg组Cu-ZnSOD和CAT的活力显著降低,差异有统计学意义(P<0.01),而GSH-Px的活力和LPO的含量显著升高,差异有统计学意义(P<0.01)。与对照组比较,雌性、雄性大鼠0.2、0.1mg/kg组Cu-ZnSOD的活力显著升高,差异有统计学意义(P<0.01);LPO的含量显著降低,差异有统计学意义(P<0.01);GSH-Px的活力差异无统计学意义(P>0.05)。结论较高剂量的混合稀土"常乐"导致大鼠肾脏脂质过氧化反应增强,清除自由基的能力下降;较低剂量的混合稀土"常乐"能抑制自由基的产生,降低体内过氧化物的含量,提高抗氧化酶的活力。

Objective To explore the effect of low dose Changle （rare earth complex） on the antioxidation in rats kidney. Methods The activities of Cu-Zn SOD,CAT,LPO and GSH-Px in the rat kidney were determined by biochemical methods after the rats （Wistar rats） were given different doses of Changle by gavage for 6 months respectively. Results The activity of Cu-Zn SOD and CAT in the 10.0 and 20.0 mg/kg groups reduced significantly,but the activity of LPO and GSH-Px increased. The activity of Cu-Zn SOD and CAT in the 2.0,0.2 and 0.1 mg/kg groups increased,but LPO reduced. Conclusion High exposure to Changle may enhance the peroxidation,but low exposure to Changle may enhance the antioxidation in rat kidney.