水中敌草隆的叶绿素荧光检测法

Study on the Method of the Determination of Diuron in Water by Chlorophyll Fluorescence Detection

目的建立水中敌草隆的ToxY-PAM荧光检测方法。方法选择三角褐指藻作为指示生物,采用ToxY-PAM叶绿素荧光仪检测水样中敌草隆对藻液光合信号的抑制程度,测定水中敌草隆的含量。结果该方法的最低检出浓度为0.1μg/L,标准曲线线性范围为0.1～0.5μg/L,相关系数为0.9993;藻液中敌草隆浓度为0.5、0.3、0.1μg/L时的日内相对标准偏差分别是3.41%,6.15%,13.9%,日间相对标准偏差分别是3.99%,6.17%,8.48%;用自来水水样做加标回收试验,敌草隆加标水平为0.2和0.4μg/L时,回收率范围分别为85.6%～110%。87.0%～94.6%。结论该法所需设备体积小,检测速度快,灵敏度高,测试稳定性好,适合于现场监测。

Objective To established the method of the determination of diuron in water by chlorophyll fluorescence Detection. Methods Phaeodactylum tricornutum was chosen as an indicator biology. The concentration of diuron in water was determined by ToxY-PAM. Results The lowest detection concentration for diuron was 0.1μg/L. The linear range of diuron was 0.1-0.5μg/L. The correlation coefficient was 0.999 3. The RSDs within one day were 3.41%,6.15% and 13.9%,and the RSDs for different days were 3.99%,6.17% and 8.48 %,when the concentrations of diuron were 0.5 μg/L,0.3 μg/L and 0.1 μg/L respectively. The recoveries for tap water were from 85.6% to 110% and from 87.0% to 94.6% when diuron was added to 0.2 μg/L and 0.4 μg/L respectively. Conclusion The apparatus is little volume and the process of the detection was rapid. With a high sensitivity and a good stability,the method was suitable for field monitoring.