固定化胰蛋白酶制备活性乳肽研究

Study on active peptides preparation by immobilized trypsin

研究固定化胰蛋白酶水解牛乳中酪蛋白制备CPPS活性肽乳的生产工艺。采用单因素实验,初步确定固定化酶制备活性肽乳反应范围,包括底物浓度、pH、加酶量、温度、水解时间。采用正交实验的方法,对初步确定的反应条件进行优化,确定固定化酶制备活性肽乳最佳工艺条件。

The paper studied the immobilized trypsin that it hydrolyzed the milk of the casein to produce the active peptides milk. To produce the active peptides milk with optimum conditions by the single experiments, including consistence of substrate, pH, the weight of enzyme, temperature and hours. Then the optimum conditions were finally optimized and determinated by the orthogonal experiments.