苯酚-硫酸法测定银杏外种皮多糖的含量

Determination on polysaccharides from Ginkgo Biloba Sarcotesta by phenol-sulfuric acid method

目的:建立分光光度法测定银杏外种皮多糖的方法。方法:采用苯酚-硫酸法对银杏外种皮多糖含量进行测定,在单因素实验结果的基础上,进行正交实验设计,考察苯酚用量、硫酸用量和显色温度对吸光度的影响。结果:硫酸用量是影响显色的重要因素。最佳条件为5%苯酚1.2mL、硫酸4mL、显色温度30℃和显色时间20min,该方法平均回收率为99.60%,RSD=3.02%(n=5)。结论:该法快速,重现性好,适用于银杏外种皮多糖的含量测定。

Objective： To develope a spectrophotometry method for the determination of polysaccharides from Ginkgo Biloba sarcotesta. Methods： The content of polysaccharides from Ginkgo biloba sarcotesta was determined by the method of phenol-sulfuric acid. On the basis of single factor tests, orthogonal experiment design method was applied to analyze the influence of factors including the dosis of 5% phenol, the dosis of sulphuric acid and temperature on the absorbance. Results： The resultes showed that the dosis of sulphuric acid was an important factor which affect the absorbance.The optimal condition was obtained. The dosis of 5% phenol is 1.2 mL, the dosis of sulphuric acid is 4 mL, temperature is 30 ℃ and time is 20 min. The averagerecovery was 99.60%, and the relative standard deviation was 3.02%. Conclusion： The method was rapid with good reproducibility, It could be used to determine the content of polysaccharides from Ginkgo Biloba sarcotesta.