刺血疗法对实验性脑缺血大鼠相关脑区HSP70mRNA的影响

The Affection of HSP70mRNA in Cerebral Cortex and Hippocampus Part of Experimental Ischemia Rat with Pricking Blood Therapy on Emergency Points

目的探讨刺血疗法对实验性脑缺血再灌流大鼠相关脑区(大脑皮层和海马区)HSP70 mRNA的影响。方法复制大鼠脑缺血再灌注模型,并取相应穴位刺血,检测HSP70 mRNA含量。结果大鼠缺血后刺血组大脑皮层和海马区HSP70 mRNA表达明显增加。结论刺血可促进脑内HSP70 mRNA表达,通过HSP70调节细胞膜钙离子通道而达到神经保护作用。

Objective： To observe the affection of HSP70mRNA in cerebral cortex and hippocampus part of experimental ischemia and refused rat with pricking blood therapy on emergency points. Methods： Tied up the rat＇s carotid artery of both side to make the cerebral ischemia and refused model, with RT- PCR method to check the HSPTOmRNA in different time of refuslon, calculate the relative amount. Results： HSPTOmRNA of cerebral cortex and hippocampus part had the dynamic ascending in pricking blood therapy on emergency points group. Conclusion： Pricking blood therapy on emergency points can promote the expression of HSP70mRNA in some brain area which can regulate calcium passage on cell membrane to conserve the nerve.