高效液相色谱法测定脑塞通丸中人参皂苷Rg_1、Re、Rb_1的含量

Determination the Ginsenoside Rg_1、Re、Rb_1 in Naosaitong Pills by HPLC

目的:建立脑塞通丸的含量测定方法。方法:采用高效液相色谱法。色谱柱:Agilent Technologies ZORBAX Extend- C_(18)(250 mm×4.6 mm,5μm),流动相:乙腈-0.05%磷酸溶液梯度洗脱,流速:1.0 ml·min^(-1),检测波长:203 nm,柱温:35℃。结果:人参皂苷Rg_1在8.516～425.8μg·ml^(-1)范围内线性关系良好(r=0.9999),平均加样回收率为97.6%,RSD=1.7%(n =6);人参皂苷Re在20.06～1003μg·ml^(-1)范围内线性关系良好(r=0.9997),平均加样回收率为97.6%,RSD=2.0%(n= 6);人参皂苷Rb_1在19.456～972.8μg·ml^(-1)范围内线性关系良好(r=0.9997),平均加样回收率为96.8%,RSD=1.3%(n= 6)。结论:该方法简便易行,结果准确可靠,可适用于脑塞通丸的质量控制。

Objective： To set up a method to determine the Ginsenoside Rg1 .Ginsenoside Re.Ginsenoside Rb1 in Naosaitong pills. Method： The Agilent Technologies ZORBAX Extend-C18 （250 mm × 4.6 mm, 5μm）was used. The mobile phase was acetonitrile- 0.05% phosphoric acid solution gradient elution. The column temperature was 35 ℃, the wavelength for detection was at 203 nm,flow rate was 1.0 ml·min^-1 Result： The calibration curves of ginsenoside Rg1 and ginsenoside Re and ginsenoside Rb1 were linear in the range 8. 516 - 425.8 μg·ml^-1 （ r = 0. 999 9） and 20.06 - 1003 μg·ml^-1 （ r = 0. 999 7 ） and 19. 456 - 972.8 μg·ml^-1 （ r = 0. 999 7 ） respectively ; the average recoveries were 97.6% （ RSD = 1.7% , n = 6 ） for ginsenoside Rg1 and 97.6% （ RSD = 2.0% , n = 6 ） for ginsenoside Re and 96.8% （ RSD = 1.3% , n = 6 ） for ginsenoside Rb1.Conelusion ： The method is simple, accurate and suitable for its assaying.