抗菌肽在棉花、烟草组织培养中的应用初探

Preliminary Exploration of the Application of Antibacterial Peptide in Cotton and Tobacco Tissue Culture

经0．1％氯化汞处理5min后，用无菌水冲洗3～5次，再用0．12mg／mL抗菌肽溶液浸泡棉花去壳种子能有效地防止棉花组织培养中常见的真菌污染，且对棉花根系的发育没有影响，同时在愈伤诱导阶段使用抗菌肽对棉花愈伤没有影响。在烟草组织培养中使用抗菌肽对愈伤及芽和芽生根没有影响，单独使用抗菌肽对细菌的杀菌效果没有头孢菌素好，但是抗菌肽与头孢菌素配合使用能有效地防止细菌污染。经初步研究抗菌肽可以应用于棉花、烟草组织培养，并对组织培养中的污染有良好的防治效果。

The treatment of peeled cotton seeds by 0.1% mercuric chloride for 5 min, washed with sterile water 3 - 5 times, and then immersed in 0.12 mg/mL antibacterial peptide liquid, was effective in preventing most common fungal contamination of the cotton tissue culture, and did not effect the development of cotton roots. The using of anti-bacterial peptide in callus induction stage showed no impact on cotton callus. The using of antibacterial peptide in the tobacco tissue culture showed no impact on callus ,shoots and shoots regeneration. Individual treatment by antibacterial peptide for the bacteria, the bactericidal effect was less than that by cephalosprin, but the joint treatment of anti-bacterial peptide and eephalosprin could prevent bacterial contamination effectively. In this preliminary study antibac-terial peptide could be applied in cotton and tobacco tissue culture present good performances in fungal and bacterium contamination control.