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维甲酸对新生大鼠高氧性肺损伤转化生长因子-β1表达的影响

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摘要 目的:探讨高氧性肺损伤新生大鼠肺损伤过程中转化生长因子-β1(transforming growth factor-β1,TGF-β1)的表达情况,并观察维甲酸(retinoic acid,RA)的干预效果,以期为临床防治高氧性肺损伤提供一条新的有效途径。方法:80只出生12h内的清洁级Sprague-Dawley(SD)大鼠作为研究对象。随机分成4组(每组20只):A组:空气对照组;B组:空气+RA组;C组:高氧组;D组:高氧+RA组。14天时观察大鼠的一般状况、体质量、肺湿重/干重值;光镜观察各组大鼠肺组织病理形态学;RT-PCR方法检测TGF-β1mRNA水平;免疫组织化学染色方法检测TGF-β1蛋白表达。结果:14天时高氧组体质量明显减轻,肺组织肺湿重/干重比值、TGF-β1mRNA及蛋白表达水平显著高于其他各组(P<0.05);高氧+RA组14天时病理改变减轻,肺湿重/干重值、TGF-β1mRNA及蛋白表达水平较高氧组明显降低(P<0.05),与空气对照组及空气+RA组相比差异无显著性(P>0.05)。结论:高氧性肺损伤新生大鼠TGF-β1表达增加,RA早期干预可以通过下调TGF-β1的表达对高氧性肺损伤具有改善作用。
作者 郦银芳 李海浪
机构地区 东南大学医学院附属中大医院儿科
出处 《南京医科大学学报（自然科学版）》 CAS CSCD 北大核心 2008年第6期794-797,共4页 Journal of Nanjing Medical University(Natural Sciences)
关键词 维甲酸 转化生长因子-Β1 高氧 肺 纤维化 新生大鼠
分类号 R329.26 [医药卫生—人体解剖和组织胚胎学]
  • 相关文献

参考文献9

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二级参考文献13

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南京医科大学学报（自然科学版）
2008年 第6期

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