中草药白花蛇舌草等对实验性大肠癌作用机制的研究

Study on experimental large intestine cancer function,such as tongue grass of long-noded pit viper of Chinese herbal medicine

目的探讨白花蛇舌草等中药在体内对大肠癌的抑制作用和抑瘤机制。方法以大肠癌裸鼠移植瘤为动物模型,观察肿瘤的生长速度,免疫组化法及肠道菌群培养技术观察大肠癌裸鼠移植瘤PCNA表达及阳性细胞密度、肿瘤坏死因子和白介素6的表达以及肠道菌群的培养。结果白花蛇舌草和仙鹤草用药组肿瘤生长率小于对照组,达50.86%,微生态调节剂组低于用药组;在肠道菌群及免疫调节能力上用药组优于对照组,微生态调节剂组优于用药组。结论白花蛇舌草和仙鹤草在体内能显著抑制大肠癌的生长。

Objective To explore the inhibition of Oldenlandia diffusa in experimental colorectal carcinoma and its antitumor mechanisms. Methods Transplanted the tumour as animal＇s model with the naked mouse of large intestine cancer,observed the speed of growth of the tumour,immune group skill and intestinal fungus group train technology to observe the naked mouse of large intestine cancer and transplant and slip away PCNA expressing and positive cell＇s density,downright bad factor of the tumour and lying between the plain expression of 6 and cultivation of the intestinal fungus group in vain. Results Long-noded pit viper tongue grass and hairyvein agrimony used medicine group tumour growth rate was lower than the contrast group, up to 50.86%. Ecology regulate pharmaceutical group was lower than the medicine group;The group was superior to contrasting the group using medicine on the intestinal fungus group and immune adjustment ability, the little ecology regulates the pharmaceutical group was superior to using medicine group. Conclusion The tongue grass of long-noded pit viper and hairyvein agrimony can suppress the growth of the large intestine cancer notably in the body.