不同发酵条件对黑曲霉利用玉米粉生产柠檬酸的影响

Effect on citri acid production by Aspergillus nigerutilizing corn meal as raw material in different fermentation conditions

研究了不同发酵条件对黑曲霉07001生产柠檬酸的影响。结果表明,以经过α-淀粉酶液化后的玉米粉作为主要原料,初始糖含量调整为15%(W/V),补充0.9%(W/V)尿素作为氮源,加入KH2PO40.1%(W/V)、MgSO40.25%(W/V),初始pH值调整为6.7,34℃发酵72 h左右,可以得到比较理想的发酵结果。

Effects on citri acid production by Aspergillus niger 07001 in different fermentation conditions were studed in details. The results showed that the perfect citri acid production could be obtained by the medium made of corn meal fluidified by α-amylase including 15%（W/V） original sugar, 0. 9% （W/V） urea, 0. 1% （W/V） KH2PO4, 0. 25% （W/V） MgSO4 ,at pH 6.7, fermentation temperature 34 ℃, fermentation times 72 h.