Ha-ras和Ki-ras癌基因转染和细胞对小鼠细小病毒杀伤敏感性的相关性(英文)

Correlation between Ha-ras or Ki-ras oncogene transfection and sensitization of cells to killing by minute virus of mice

为研究Ha－ras和Ki－ras癌基因转染和细胞对小鼠细小病毒（MVM）杀伤敏感性间的关系，两个细胞株，即Ki－ras癌基因转化细胞株DT和Ha－ras癌基因转化细胞株REF4-3，被用来作为研究材料．体外细胞集落形成率和细胞在裸小鼠体内的成瘤能力测定显示，和对照细胞NIH／3T3相比，REF4－3和DT对MVM的杀伤作用更敏感．同时，DNA杂交和蛋白免疫沉淀实验结果也显示，在REF-3和DT细胞中，无论是MVM的DNA增殖能力还是其NS－1蛋白的表达能力，均较在其对照组NIH／3T3细胞中高很多．FACA测定也显示，在感染MVM30h后，S期细胞的比例在REF4－3和DT细胞中都有增加，而在NIH／3T3细胞中却略微减少．通过PCR方法也可发现，在受到MVM抑制的REF-3肿瘤中仍可检测到MVM的DNA．

To Investigate the relationship between Ha-ras or Ki-ras oncogene transfection and sensitizatlon of cells to the killing effect by the minute virus of mice (MVM), a parvovirus,two cell lines, Ki--ras oncogene transformed cell line DT, and Ha-ras oncogene transformed cell line REF4-3 .were used. Measurement of cell coloning efficiency in in vitro and tumorigenic ability in nude mice showed that REF4-3 and DT were more sensitive to parvovirus MVM than their control, NIH/3T3. Furthermore, by DNA hybridization and protein immunoprecipitation, we found that both amplification of MVM DNA and expression of MVM NS-1 protein were also much higher than those in NIH/3T3. FACA showed that 30 hours after infection with MVM, the percentage of S-phase cells increased in REF4-3 and DT,whereas it decreased slightly in NIH/3T3. By polymerase chain reactions, we also detected MVM DNA in MVM-suppressed REF4-3 tumor.