摘要
对我国6个地区和日本3个地区灰飞虱的Wolbachia感染率,用PCR技术进行了检测,结果表明中国的辽宁、北京、上海和云南的灰飞虱的Wolbachia感染率均接近100%;四川为59.6%;而宁夏为0.各地区灰飞虱间的交配实验证明了Wolbachia引起的细胞质不亲和(CI)现象的存在.Wolbachia16SrDNA的部分测序分析表明,上海、云南和日本出云的灰飞虱感染的同为Wolbachiapipientis。这一结果为掌握灰飞虱-Wolbachia的共生关系,利用灰飞虱体内共生菌防治水稻条叶枯病毒病提供依据.CI现象的研究,可以在多种虫媒传播的动植物疾病防治上应用,开辟出一条基因工程防治的新途径.
Wolbachia is a group of endosymbionts that habor diverse insect species. These microorganisms are maternally inherited and cause developmental defects of their hosts, including a phenomenon called cytoplasmic incompatibility (CI). In this paper, Wolbachia infections in the small brown planthopper, Laodelphax striatellus Fallen,from six locations in China and three from Japan were studied. The results had shown that the infection rates of Wolbachia are 100% for planthoppers from Liaoning, Beijing, Shanghai, and Yunnan,59. 6% for that of Sichuan, and none in Ningxia. The presence of CI was confirmed by mating experiments with planthopper populations from the above-mentioned locations in China.Sequencing of Wolbachia 16S rDNA for planthopers from Shanghai, Yunnan, and Izumo (Japan ) indicated that these Wolbachia strains harboring different planthopper populations belong to the same species,ie., Wolbachia pipientis.
出处
《复旦学报(自然科学版)》
CAS
CSCD
北大核心
1997年第5期500-506,共7页
Journal of Fudan University:Natural Science
基金
美国McKnight Foundation资助
上海市生命科学中心资助