反刍动物精料补充料中肉骨粉快速检测近红外光谱法

NIRS Method for Determination of Meat and Bone Meal Content in Ruminant Concentrates

为探讨利用近红外漫反射光谱技术快速定量分析反刍动物精料补充料中肉骨粉含量的可行性,收集了我国常用的、具有代表性的精料补充料225个和肉骨粉75个,按照不同的肉骨粉含量（0.5%-35%）制备样品225个,其中135个为定标集,90个作为独立的验证集。用光栅型近红外光谱仪进行光谱扫描,选择合适的光谱预处理方法和光谱范围,采用改善的偏最小二乘法（MPLS）建立了定量分析模型,相关系数为0.979,标准差为1.522%,交互验证标准差为1.582%。用90个独立的验证样品对所建立的定量分析模型进行验证,相关系数为0.972,预测标准差为1.764%,RPD值为5.99。结果表明利用近红外反射光谱法可以分析精补料中肉骨粉的含量,对于丰富我国动物源性饲料的快速检测手段,具有实际应用意义。

Feed contaminated with MBM is commonly accepted as the main transmission carrier of bovine spongiform encephalop- athy （BSE）. To prevent BSE many countries have banned MBM as a feed ingredient. In the People＇s Republic of China, the ban was first applied to ruminant feed. In order to investigate the feasibility of near infrared diffuse reflectance spectroscopy method for rapidly quantitative determination of meat and bone meal content in ruminant concentrates, 225 representatively commercial ruminant concentrates samples and 75 meat and bone meal （including cattle, sheep, pig and poultry meat and bone meal） samples were collected in the People＇s Republic of China. Two hundred twenty five ruminant concentrates samples of adulterated meat and bone meal （0.50%-35%） were prepared including 135 calibration samples and 90 independent validation samples. For the calibration set samples, 3 samples were prepared at each concentration. For validation set samples, 2 samples were prepared at each concentration. Any one commercial ruminant concentrates was used once only. The spectra were scanned by raster near infrared diffuse reflectance spectroscopy instrument, and the effect of spectrum pretreatment methods （mathematic pretreatments and scatter correction） and spectrum region （visible and NIR） on the calibration results was considered. The calibration equation was established by modified partial least squares method. The result showed that the calibration gave re of 0. 979, a standard error of calibration （SEC） of 1. 522% and a standard error of cross validation （SECV） of 1. 582% . The 90 independent validation samples were used to validate the quantitative equation. The r^2, a standard error of prediction （SEP） and ratio of performance to standard deviation （RPD） were 0. 972, 1. 764% and 5.99 respectively. The results of this study indicated that near infrared diffuse reflectance spectroscopy method could provide rapidly quantitative prediction for meat and bone meal percent in ruminant concentrates. This method was significant in practice for enriching the rapidly quantitative methods of determining animal feed materials.