PCV2、PPV、PRV、PRRSV和CSFV复合PCR的应用研究

Application of the Multiplex PCR Detecting PCV2,PPV,PRV,PRRSV and CSFV

采用建立的PCV2-PPV-PRV-PRRSV-CSFV复合PCR检测方法,对山东省不同地区送检的32份临床疑似病料进行PCR检测。结果表明:32份样品中,PCV2、PPV、PRV、PRRSV和CSFV感染的阳性率分别为25.00%、0%、34.38%、71.88%和3.13%;共检出25份阳性病料,阳性率为78.13%,其中PRV-PRRSV双重感染的比例为31.25%,PCV2-PRV-PRRSV三种病原体混合感染的比例为25.00%;用单项PCR检测作对照,结果两者符合率为100%,表明该复合PCR检测方法具有较高的敏感性,可以作为临床上猪圆环病毒2型感染、猪细小病毒病、猪伪狂犬病、猪繁殖与呼吸综合征和猪瘟的病原学快速诊断方法。

The established multiplex PCR of PCV2-PPV-PRV-PRRSV-CSFV was used to detect thirty-two clinical cases.These cases were collected from different areas of Shandong Province for the PCR detection.The results showed that the infection rate of PCV2,PPV,PRV,PRRSV and CSFV was 25.00%,0%,34.38%,71.88% and 3.13%,respectively.Twenty-five positive cases were detected and positive rate was 78.13%.The infection ratio of PRV-PRRSV double pathogens was 31.25% and the infection ratio of PCV2-PRV-PRRSV three pathogens was 25.00%.The single PCR was used as a control,and the result showed that the multiplex PCR accorded with the single PCR about 100%.It indicated the multiplex PCR had higher sensitivity.The multiplex PCR can be used as a rapid etiology diagnostic method to detect clinical diseases of porcine circovirus type 2 infection,porcine parvovirus disease,swine pseudorabies,porcine reproductive and respiratory syndrome and classical swine fever.