聚合酶链反应检测慢性乙肝患者血清唾液及尿液中HBV DNA

DETECTION OF HBVDNA IN SERUM,SALIVAAND URINE BY PCR IN PATIENTSWITH CHRONIC HEPATITIS B

采用聚合酶链反应检测１００例慢性乙肝患者血清、唾液及尿液中ＨＢＶＤＮＡ结果：血清中ＨＢＶＤＮＡ阳性率最高，为８０％，显著高于唾液及尿液（Ｐ值均小于０．０１）。ＨＢｅＡｇ（＋）、抗－ＨＢｅ（－）组与ＨＢｅＡｇ（－）、抗－ＨＢｅ（＋）组比较，前者血清及唾液中ＨＢＶＤＮＡ检出率明显高于后者（Ｐ值分别小于０．０１和０．０５）。ＨＢｅＡｇ（＋）、抗－ＨＢｅ（－）组与其他模式组比较，前者只有血清中ＨＢＶＤＮＡ阳性显著高于后者（Ｐ＜０．０１），结果提示：①ＨＢＶＤＮＡ阳性率与ＨＢＶＭ存在状态密切相关。②评价乙肝患者是否存在ＨＢＶ复制及传染性，不能单纯依靠ＨＢＶＭ，二者互补检测将有助于提高乙肝诊断率及ＨＢＶ是否复制。

The HBVDNA in serum,saliva and urine were detected by polymerase chain reaction (PCR)in 100 patients with chronic hepatitis B.The results were as follows:the positivity rate of serum HBVDNA was the highest (86%),which abviously higher than that in saliva and urine (P〈0.01);the positivity rates of serum and saliva HBVDNA in HBeAg(+) and anti HBe( ) group were notably higher than those in HBeAg( )and anti HBe(+) group.As compared the positivity rates in the HBeAg(+)and anti HBe( ) group with the other HBV M groups the positivity rate of HBVDNA only in serum was significantly higher(P〈0.01).These results suggest that (1) there are close relations between the positivity rate of HBVDNA and state of HBV M,(2) to appraise whether the patients are infected and the HBV are in replication,both HBV M and HBVDNA should be detected in the same time;It would be helpful for improving the confirmed diagnosis rate of hepatitis B and judging whether the HBV is in replication.