摘要
目的探讨血管内皮生长因子-C(VEGF-C)在不同类型胃癌细胞株及组织中的表达及其与临床病理之间的关系。方法应用反转录-聚合酶链反应(RT-PCR)、Westernblot分别检测VEGF-CmRNA及其蛋白在胃癌细胞株中的表达,对53例胃癌及癌旁组织中VEGF-C的表达采用免疫组织化学SP法染色检测,Ⅷ因子抗体及Ⅳ型胶原抗体双重免疫组织化学染色(SABC)法进行微淋巴管染色及微淋巴管计数。结果在胃癌细胞株中均可检测到VEGF-CmR-NA及蛋白的表达,胃癌组织中,VEGF-C蛋白的表达与淋巴结转移、病理分期有关(P<0.05);淋巴管密度与VEGF-C蛋白的表达、淋巴结转移、病理分期有关(P<0.05)。结论VEGF-C表达与胃癌淋巴结转移密切相关,并有可能参与了胃癌淋巴管的形成。
Objective To study the relation between clinicopathological characteristic with the expression of vascular endothelial growth factor (VEGF-C) in different type of gastric cancer cell lines. Methods The expression of VEGF-C mRNA and protein in gastric carcinoma cell lines was examined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot respectively. Fifty and three cases' expression of VEGF-C in gastric cancer and in the para-cancer tissue were detected by immunohistochemical methods. Double immunohistostaining with Ⅷ factor antibody and Ⅳ collagen antibody was used to stain and count the micro-lymphatic. Results The expression of VEGF-C mRNA and its protein in all gastric carcinoma cell lines could be detected. Expression of VEGF-C in gastric cancer associated with lymphatic metastasis and pathological staging ( P 〈 0.05 ). Lymphatic density also concerned with the expression of VEGF-C, lymphatic metastasis and pathological staging ( P 〈 0. 05 ). Conclusion VEGF-C may play an important role in lymphatic metastasis of gastric cancer. , and could involve in formation of lymphangiogenesis.
出处
《新乡医学院学报》
CAS
2008年第4期353-356,共4页
Journal of Xinxiang Medical University
关键词
胃癌
血管内皮生长因子-C
淋巴管
淋巴结转移
gastric cancer
vascular endothelial growth facto-C
lymphatic vessel
lymph node metastasis