生物絮凝剂产生菌的筛选及培养条件优化

Screening of microbe producing flocculant and optimization on its cultural conditions

从林下土壤中筛选得一株产絮凝剂的细菌XMX-1(其产生的絮凝剂命名为XMX-1F);研究结果表明:玉米淀粉、玉米浆是廉价的碳氮源;通过乙醇提取的方法,从发酵液离心后的上清液中获得絮凝剂粗产品7.685 g/L;通过蒽酮反应、紫外扫描以及红外扫描等多种方法手段分析确认XMX-1F是以多糖为主的高分子絮凝剂,多糖含量为77.87%;在实际废水处理方面,通过XMX-1F对模拟洗煤废水的应用研究表明,其应用到处理实际洗煤废水中的前景是可观的.

A strain was screened from soil, and named XMX-1（the bioflocculant produced byXMX-1 named XMX-1F）, which has the highest flocculating activity. Cornstarch and the corn steep liquor were chosen as carbon and nitrogen sources. 7.685 g/L XMX-1F raw product was obtained from the supematant by using ethanol. The XMX-1F is mainly composed of polysaccharide, which was determined by Anthrone reaction, ultraviolet spectroscope method and Infrared spectra. The polysaccharide content is 77.87%. In the treatment of high SS waste water, XMX-1F shows high flocculating activity. Study of XMX-1F flocculating coalwashing wastewater indicates the actual application potential of XMX-1F.