基质金属蛋白酶MMP-3及其组织抑制剂TIMP-3在子宫内膜异位症中的表达

Expression of matrix metalloproteinase-3 and tissue inhibitor of metallo proteinases-3 in endometriosis

目的:检测基质金属蛋白酶MMP-3及其组织抑制剂TIMP-3在内异症患者的腹膜红色病变和卵巢巧囊两种异位内膜组织及同期在位内膜组织中的基因表达,探讨两者与内异症发生、发展的关系。方法:应用RT-PCR法分别检测38例内异症患者的异位内膜组织(腹膜红色病变16例和卵巢巧囊22例)、20例内异症患者的同期在位内膜,以25例非内异症患者的子宫内膜组织作为对照,比较不同组织中MMP-3、TIMP-3 mRNA相对表达量的差异,并将结果进行统汁学分析。结果:①两种异位内膜组织中MMP-3和TIMP-3 mRNA的差异表达:腹膜红色病变中的MMP-3 mRNA含量高于卵巢巧囊及对照组子宫内膜的表达(P<0.05);卵巢巧囊中TIMP-3 mRNA的含量高于腹膜红色病变及对照组子宫内膜(P<0.05);腹膜红色病变中MMP-3/TIMP-3显著高于卵巢巧囊及对照组子宫内膜(P<0.05)。②在位内膜组织中MMP-3和TIMP-3 mRNA的表达:内异症患者的同期在位内膜中MMP-3mRNA的含量高于对照组子宫内膜(P<0.05),TIMP-3 mRNA的含量与对照组子宫内膜相比,差异无显著性(P>0.05);在位内膜组织中MMP-3/TIMP-3 mRNA高于对照组子宫内膜(P<0.05)。结论:①MMP-3 mRNA在疾病早期的腹膜红色病灶中高表达,使异位内膜的侵袭性增强,与内异症的发生有关;TIMP-3在卵巢巧囊中表达上调,能抑制病灶的活动,故内异症发展至巧囊时侵袭力不及早期红色病变。②在位内膜中MMP-3 mRNA高表达,可能是内异症发生的源头。

Objective：To investigate the genic expression of matrix metalloproteinase-3（MMP-3）and tissue inhibitor of metallo proteinases-3（TIMP-3）in eutopic and ectopic endometrial tissues of endometriosis and to explore the correlation between the two factors and the biological property of endometriosis.Methods：The expressions of MMP-3 and TIMP-3 on the level of mRNA were detected in 38 cases of ectopic endometrium of endometriosis（16 cases of red peritoneum lesion,22 cases of chocolate cyst of ovary）,20 cases of eutopic endometrium of endometriosis,and 25 patients with uterine fibroids as controls by RT-PCR.The differences in mRNA expression of the MMP-3,TIMP-3 in different tissues were compared and the results were analyzed statistically.Results：①The levels of MMP-3 mRNA in the group of red peritoneum lesion were elevated when compared with the groups of chocolate cyst of ovary and controls（P〈0.05）;The expressions of TIMP-3 mRNA in the group of chocolate cyst of ovary were rise when compared with the groups of red peritoneum lesion and controls（P〈0.05）;The rate of MMP-3 mRNA/TIMP-3 mRNA in the group of red peritoneum lesion were striking rise（P〈0.05）.②In the group of eutopic endometrium of endometriosis,the expressions of MMP-3 mRNA were higher than those in controls（P〈0.05）,while the expressions of TIMP-3 mRNA were not（P〉0.05）;In the group of eutopic endometrium,the expressions of MMP-3/TIMP-3 mRNA were higher than those in controls（P〈0.05）Conclusion：①The persistently exaggerated expression of MMP-3 mRNA may plays an important role in the invasion at the early stage of endometriosis;The higher expression of TIMP-3 may contribute to decline the activity of the disease in its late stage.②The exaggerated expression of MMP-3 mRNA in eutopic endometrium of endometriosis may be the origin of endometriosis＇s occurrence.