扶正抗癌方药物血清对人肝癌细胞增殖的影响

Serum-pharmacological Effects of Fuzheng Kang'ai Decoction (FZKAD) on the Proliferation of Hepatocarcinoma Cells

目的：阐明扶正抗癌方的抗癌机制。方法：以不同剂量灌胃给药后，不同时间采集大鼠血清、处理体外培养的人肝癌细胞ＳＭＭＣ７７２１，用ＭＴＴ法和氚标胸嘧啶掺入法观察细胞增殖能力。结果：扶正抗癌方２次给药后１小时和２小时药物血清处理细胞，ＭＴＴ转化率和＾３Ｈ－ＴｄＲ掺入率明显下降。结论：扶正抗癌方具有明显的抑制人肝癌细胞增殖作用。

Aim: In order to ascertain the anti-carcinoma mechanism of FZKAD. Metheds: FZKAD drug-serum was derived at different time from rats which were orally preadministrated with different-dose FZKAD. The effects of the serum on human hepatoma cells (SMMC7721) were observed by MTT assay and H-TdR incorporating method. Results: After treating with the drug-serum derived at 1h and 2h separately after administrating twice with FZKAD (1. 6g/kg, 8.0g/ kg ), the MTT transformative and 3H-TdR incorporative rate were decreased significantly. Conclusion: FzkaD had remarkable inhibitory effects on proliferation of human hepatoma cells. It might be one of the anticarcinoma mechanisms of FZKAD.