健康人支气管肺泡灌洗液中细胞、免疫球蛋白和补体含量以及吸烟的影响

THE DETECTION OF CELLS,IMMUNOGLOBULIN AND COMPLEMENT IN BRONCHOALVEOLAR LAVAGE FLUID AND SMOKING INFLUENCE ON THEM

目的：了解健康人支气管肺泡灌洗液中细胞、免疫球蛋白和补体含量以及吸烟对其影响。方法：采用Ｒｅｙｎｏｌｄｓ介绍的支气管肺泡灌洗术灌洗健康不吸烟者１６例、健康吸烟者１１例的下呼吸道后，检测灌洗液中细胞及非细胞成分含量。结果：灌洗液中细胞总数，吸烟组高于不吸烟组（Ｐ＜００１），分类均以巨噬细胞为主，淋巴细胞吸烟组低于不吸烟组（Ｐ＜００５）；灌洗液中ＩｇＡ／Ａｂ比例比血清高（Ｐ＜００１），ＩｇＡ／Ａｂ吸烟组低于不吸烟组（Ｐ＜００５），ＩｇＧ／Ａｂ两组之间无差异（Ｐ＞００５）；补体各种成份均能检出，但以Ｃ５为主。结论：支气管肺泡灌洗术可作为观察肺免疫状况窗口。吸烟对健康者下呼吸道免疫功能有较大影响。

OBJECTIVE: To detect cells, immunoglobulin and complements in broncho-alveolar fluid and study influence of smoking on them. METHOD: 16 healthy non-smokers and 11 healthy smokers were conducted broncho-alveolar lavage, which was introduced by Reynolds. The cellular and non-cellular components in the lavage fluid were examined. RESULTS: The total amount of cells (mainly macrophages) in smoking group was higher than that in non-smoking group (P<001), but lymphocytes in smoking group were less than in non-smoking group (P<005). The IgA/Ab ratio in lavage fluid was higher than that in serum (P<005) and was lower in smoking group than that in non-smoking group (P<005). But IgG/Ab ratio had no difference between the two groups (P>005). The complement component was detectable, but mainly C3. CONCLUSION: The examination of broncho-alveolar lavage fluid can indicate immunological status. Smoking produces influence on immunological function of lower respiratory tract.