摘要
目的测定JAK家族成员以及SOCS1在运动后心肌的表达，以探讨它们在运动心肌JAK／STAT信号通路中可能的相互作用。方法雄性SD大鼠进行递增负荷跑台运动，采用免疫组化法观察运动后0、1、3、5、、12和24h心肌JAK1、JAK3和SOCS1的表达。结果运动后即刻大鼠心肌细胞JAKl的表达显著加强，并持续到运动后24h；而大鼠心肌细胞JAK3及SOCS1的表达在运动后1～5h显著降低，两者在运动后12h恢复到安静水平。结论急性运动中，JAK1和JAK3对运动心脏功能的调节可能作用不同，SOCS1可能参与了运动心肌JAKs／STAT3信号通路的调节过程。
Objective To evaluate Janus kinase（JAK）s and SOCS1 expression in myocardium induced by acute exercise in order to understand the possible regulating mechanism of SOCS1 in JAK/STAT signaling pathway in exercised heart. Methods Male Sprague-Dawley rats were submitted to continuous graded treadmill running, then were sacrificed at 0, 1, 3, 5, 12 and 24 h respectively after the exercise. JAK1, JAK3 and SOCS1 expressions in myocardium were determined with immunohistochemistry. Results JAK1 expression significantly increased imme- diately after running in comparison with control group, peaked at 1 N 5 h, and remained high at 24 hr after exer- cise; in contrast, JAK3 and SOCS1 expressions significant decreased from 1 h to 5 h after running, then gradually back to sedentary level 12 h after exercise. Conclusion Acute exercise induced different responses of JAK1 and JAK3, which indicated the different functions of JAKs in exercised heart, SOCS1 may involve in regulating JAK/ STAT3 signal pathway in the exercised heart.
出处
《基础医学与临床》
CSCD
北大核心
2008年第6期575-578,共4页
Basic and Clinical Medicine
基金
北京市重点实验室开放实验室课题资助项目(2007-1)
