Wnt-1重组腺病毒对人表皮干细胞分化的影响

Influence of Wnt-1 recombinant adenovirus on differentiation of human epidermal stem cells

目的了解Wnt-1重组腺病毒对人表皮干细胞（ESC）分化趋势的影响。方法将Wnt-1重组腺病毒导入人ESC（实验组）后，检测细胞直径及增殖情况、ESC多种分子标记物的表达、培养上清液中基质金属蛋白酶2（MMP-2）和MMP-7的含量。以正常ESC为对照组。结果对照组、实验组ESC直径接近（P〉0．05）。对照组ESC为（1．18±0．10）×10^5个／mL，实验组为（1．45±0．09）×10^5个／mL，后者明显高于前者（P〈0．05）。2组ESC分子标记物角蛋白5（K5）、K6、K7、K8、K14、CD44、癌胚抗原、雌激素受体、孕激素受体的表达量组间比较，差异均无统计学意义（P〉0．05）；对照组K10表达量为0，实验组为（60±3）％；对照组K18为（13．8±1．7）％，实验组表达明显增强[（34．3±2．1）％，P〈0．05]；对照组K19为（24．4±1．5）％，实验组减弱至（17．1±1．8）％（P〈0．05）。实验组ESC培养上清液中MMP-2和MMP-7含量均明显高于对照组（P〈0．01）。结论Wnt-1重组腺病毒具有诱使人ESC向腺样上皮细胞分化的趋势。

Objective To observe the influence of Wnt-1 recombinant adenovirus on differentiation tendency of human epidermal stem cells. Methods Wnt-1 recombinant adenovirus was transduced into hESCs （E group） ,while normal hESCs were used as control （C）group. The diameter, proliferation, and labeling molecular expression of hESC were determined. The content of MMP-2 and MMP-7 in supernate were also assayed. Results There was no obvious difference in diameter of hESC between two groups. The density of hESC in E group was （ 1. 45 ± 0. 09）×10^5/mL, which was obviously higher than that in C group [ （1.18 ± 0. 10）×10^5/mL, P 〈0.05 ]. There were no obvious differences in expression of markers between two groups,including keratin 5 （ K5 ） , K6, K7, K8, K14, CD44, carcinoembryonic-like antigen （ CEA ） , ER, PR（ P 〉0.05） ,while the expression of K 10 was different among groups[ （60 ±3）% in E group, 0 in C group] ,also K18 [ （34.3 ±2. 1）% in E group, vs. （13.8 ±1.7）% in C group, P 〈0.05], and K19 [（17.1 ±1.8）% in E group .vs. （24.4 ±1.5）% in C group, P 〈0.05].The contents of MMP-2 and MMP-7 in E group were higher than those in C group （ P 〈0.01 ）. Conclusion Wnt-1 recombinant adenovirus can induce the differentiation of hESCs to glandular epithelium-like cells.