摘要
目的检测SEPT7对胶质瘤细胞系U251的细胞周期、增殖、侵袭以及凋亡的影响。方法流式细胞术分析转染SEPT7对细胞周期的影响,Western blot检测细胞周期因子表达变化,MTT法和Annexin V法评价SEPT7对U251细胞的增殖活性和凋亡的影响,Martrigel三维立体培养分析转染SEPT7后U251细胞侵袭能力的变化。结果SEPT7使U25I细胞G0/G1期阻滞,S期细胞比例(SPF)降低,增殖活性和侵袭能力明显受到抑制,并可诱发细胞凋亡。结论SEPT7抑制U251细胞侵袭、增殖,促进凋亡。结果提示,SEPT7是对胶质瘤具有抑制作用的基因。
Objective To investigate the effect of SEPT7 on cell cycle and proliferation, invasion, apoptosis of gliomas cells U251. Methods The cell proliferation was determined by MTT assay and flowcytometry, cell regulators were detected by western blot, cell apoptosis was detecteded with Annexin V staining and cell invasion was evaluated by motility in three-dimensional culture. Results It was found that after transfection with SEPTT, the proliferation activity of U251 cell was inhibited, cell cycle was arrested in G0/G1 phase and the S phase fraction (SPF) was lowered. SEPT7 also increased apoptotic cells and attenuated the invasive ability of U251 cell. Conclusion SEPT7 inhibited tumor growth and invasion, and induced cell apoptosis in vivo. These results reveal that SEPT7 exerted the suppressive effect on the glioma cell growth and invasion, induced apoptosis, and suggested that SEPT7 as a suppressor gene of glioma.
出处
《中华神经外科杂志》
CSCD
北大核心
2008年第6期471-473,共3页
Chinese Journal of Neurosurgery
基金
国家自然科学基金(30500523)
天津市科委科技攻关项目(06YFSZSF01100)
天津市高等学校科技发展基金(20070234)