氯沙坦及维生素E对高强度军事训练致大鼠肾损伤模型的保护作用

Protective effect of Losartan and vitamin E against military overtraining-induced kidney injury in a rats model

目的探讨氯沙坦、维生素E对于高强度军事训练致大鼠肾损伤模型的保护作用。方法40只雄性SD大鼠随机分对照组、模型组、氯沙坦组、维生素E组,除对照组外,其余组按高强度负荷累积肾损伤模型的训练方法行8周跑台训练,每周5d,每天1次,每次持续20min。观察每周末的尿液及8周训练结束时血、尿各指标改变。结果训练结束后,模型组、氯沙坦组、维生素E组的尿蛋白、尿N-乙酰-β-D-葡萄糖苷酶(NAG)、血清尿素氮(BUN)、肌酐(SCr)值均高于对照组(P<0.05),其中模型组值高于氯沙坦组、维生素E组,氯沙坦组低于维生素E组(P<0.05)。训练结束模型组、氯沙坦组、维生素E组血浆及肾组织血管紧张素Ⅱ浓度(angio-tensinⅡ,AngⅡ)高于对照组,其中氯沙坦组高于模型组(P<0.05),维生素E组与模型组无差异(P>0.05)。模型组、氯沙坦组肾组织Na+-K+-ATP酶活性低于对照组和维生素E组,其中氯沙坦组高于模型组(P<0.05),维生素E组与对照组间无明显差异(P>0.05)。结论氯沙坦能有效降低尿蛋白、NAG、BUN等,有一定的抗氧化作用。维生素E能增强训练能力,在降低尿蛋白、NAG、BUN等方面弱于氯沙坦,但抗氧化作用明显。两种药物对于高强度军训引起的肾脏损伤均有保护作用。

Objective To study the preventive and therapeutic effects of losartan and vitamin E on kidney injury induced by overtraining in a rat model. Methods Forty SD male rats were randomly divided into four groups （10 each） ： control group, model group, losartan group and vitamin E group. Rats in control group were fed with conventional diet. Rats in other three groups were forced to run on the treadmill for 8 weeks, once a day for 20 minutes and 5 days for each week, till exhaustion to induce the kidney injury. Rats in losartan group and vitamin E group were gavaged either with losartan or vitamin E everyday. Urine was collected at the end af each week, urine protein and N-acety Dglucosaminidase （NAG） were detomlined, and the serum urea nitrogen, creatinine （SCr）, angiotensin Ⅱ, Na^1-K^1 - ATP-ase activity in kidney were analyzed after 8 weeks＇ training. Results After 8 weeks＇ training, the urine protein, NAG in urine, blood urea nitrogen （BUN）, SCr were obviously increased in model group, losartan group and vitamin E group as compared with that in control group, while those in model group were higher than that in losartan group and vitamin E group （ P〈0. 05）, and those in losartan group were lower than that in vitamin E group （P〈0. 05）. The concentration of angiotensin Ⅱ of serum and kidney in model group, losartan group and vitamin E group were significantly higher than that in control group （ P〈0. 05）, and it was higher in losartan group than that in model group （P〈0.05）. The values showed no significant difference between model group and vitamin E group （P〉0. 05）. Na^1-K^1- ATP ase activity of renal tissue were obvionsly lower in model group and losartan group than that in control group and vitamin E group （ P 〈0. 05）, and itwas higher in losartan group than that in model group （P〈0. 05）, while no significant difference was found between control group and vitamin E group. Conclusions Losartan and vitamin E may protect the kidney from injury caused by high intensity training. Losartan can effectively decrease urine protein, NAG and BUN, and has some anti-oxidation effect, vitamin E is relatively less effective in anti-oxidation.