摘要
以单盲法及目测判读与检测OD值对比观察快速ELISA诊断血吸虫病的效果。单盲法检测56例日本血吸虫病人及33名健康者血清,敏感性94,6%,特异性97.0%;与其它寄生虫病(肺吸虫病)的交叉反应率为6.2%。以目测法判断与测定OD值作对比,检测58例血吸虫病人及35名健康者血清,阳性检出率分别为94.8%和96,5%,特异性分别是100%和97.1%;经χ2检验,P>0.05两法判断结果无显著性差异;与肺吸虫病和肝吸虫病均未见交叉反应。本实验进一个证明:快速-ELISA试验具有较好的诊断价值,适于现场推广应用。如以测定OD值判断结果,则使该法更具客观标准。
This article showed diagnostic effects on schistosomiasis Japonica with Q-ELISA, by read-ing with the naked eye and/or by OD value. With the single-blind method, 56 cases of schistosomiasisJaponica and 33 healthy persons were detected. Sensitivity and spectificity,ere 94. 6% and 97. 0%respectively, and the rate of cross-reaction with paragonimiasis patients was 6. 2%. Furthermore, 58cases of schistosomiasis Japonica and 35 healtby persons were tested, and the seusitivity and speci-ficity were 94. 8% and 100% with the naked eye, and 96. 5% and 97. 1% by OD value. There was no statis-tically significant difference(P>0.05) between the results with the naked eye aud by OD value. Andno cross-reaction with paragonimiasis and clonochiasis was found. The present study further indicat-ed that the Q-ELISA possessed better diagnostic value and might be applied in the fields. Bnt if byOD value, the Q-ELISA assay was more objective.
出处
《镇江医学院学报》
1997年第1期14-15,共2页
Journal of Zhenjiang Medical College
关键词
血吸虫病
快速-ELISA
单盲法
诊断
Schistosomiasis japonica
Q-ELISA
Circulating antibody
single-blind method