血管紧张素原基因多态性与深静脉血栓的相关性研究

Study on the association between polymorphisms in angiotensinogen gene and deep venous thrombosis

目的探讨血管紧张素原（angiotensinogen，ACT）基因-6G／A、-20A／C、T174M 3种基因型多态在深静脉血栓发病中的意义。方法应用PCR-限制性片段长度多态性方法对103例深静脉血栓患者和250名健康对照者进行AGT-6G／A、-20A／C、T174M基因多态性的检测，并进行基因型及等位基因频率和单倍型分析。结果-6G／AGA基因型在深静脉血栓组中的分布频率（60．2％）明显高于对照组（39．6％），经x^2检验差异具有统计学意义（P〈0．05）。单倍型分析显示病例组中-20A／-6G／174T单倍型频率高于对照组（P〈0．05）。-20A／-6A／174T单倍型频率低于对照组（P〈0．05）。-20A／C、T174M基因型在两组的分布频率差异无统计学意义。结论AGT-6G／A多态性中，GA基因型可能会增加深静脉血栓的发生。-20A／-6G／174T单倍型可能是静脉血栓的危险因素，-20A／-6A／174T单倍型可能是静脉血栓的保护因素。

Objective To investigate the association of polymorphism of angiotensinogen （AGT） - 6G/A, - 20A/C and T174M with the development of deep venous thrombosis. Methods One hundred and three patients with deep venous thrombosis （DVT group） and 250 healthy subjects （control group） were recruited in the study. The polymorphisms of angioteusinogen - 6G/A, - 20A/C and T174M were detected by PCR-RFLP. Retmlts The prevalence of GA genotype of - 6G/A in the DVT group was significantly higher than that in the control group （ P 〈 0.05 ） and the prevalence of -20A/- 6A/174T haplotype in the DVT group was lower than that in the control group（ P 〈 0.05）. There were no significant differences in the prevalence of - 20A/C and T174M polymorphism. Conclusion The GA genotypes of -6G/A may increase the development of DVT and the - 20A/- 6G/174T haplotype may be a risk factor of DVT. However, the - 20A/- 6A/174T haplotype may be a protective factor of DVT.