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普那霉素高产相关基因的筛选 被引量：2

Screening of High Pristinamycin Yield-related Genes in Streptomyces pristinaespiralis
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摘要 普那霉素是由始旋链霉菌(Streptomyces pristinaespiralis)产生的一类临床上应用的链阳性菌素类抗生素.目前,由于对引起普那霉素产量变化的分子机理知之甚少,从而大大限制了利用分子育种技术来大幅度提高该抗生素产量的研究发展.本研究利用限制性内切酶SacⅡ单酶切处理的扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术以普那霉素高产重组菌株为材料进行了抗生素高产相关基因的挖掘研究.以原始菌株ATCC25486为对照,筛选出2个仅在高产菌株中特异扩增的多态性DNA片段.进一步的DNA测序分析和同源性比较表明:1个与抗生素生物合成调控基因afsK具有高度的同源性,GenBank收录号为EU123927;另1个是脱氧核糖核酸外切酶编码基因exoSC的同源片段,GenBank收录号为EU123928.因此,这2个DNA片段是与普那霉素产量变化密切相关的新基因,这2个基因的DNA序列的变异应与普那霉素产量的增加密切相关.本研究从普那霉素高产这一生物表型出发,利用分子标记技术筛选出抗生素高产相关新基因,可进一步从基因变异方面揭示了普那霉素高产的分子机理.
作者 金庆超 金志华 徐波 李宁慧 姚善泾
机构地区 浙江大学宁波理工学院生化分院 浙江大学材料与化学工程学院
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2008年第6期581-585,共5页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金项目(No.20576122) 浙江省科学技术厅项目(No.2004C13007) 浙江省自然科学基金项目(No.Y404291)资助~~
关键词 始旋链霉菌 普那霉素 高产相关基因 筛选
分类号 Q75 [生物学—分子生物学]
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参考文献16

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同被引文献18

  • 1Wen-Tao PENG,Jin WANG,Ting WU,Jian-Qiang HUANG,Jui-Shen CHIAO,Guo-Ping ZHAO.Bacterial Type Ⅰ Glutamine Synthetase of the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32,is the Only Enzyme Responsible for Glutamine Synthesis under Physiological Conditions[J].Acta Biochimica et Biophysica Sinica,2006,38(12):821-830. 被引量：4
  • 2Hao YU,Wen-Tao PENG,Yang LIU,Ting WU,Yu-Feng YAO,Ming-Xue CUI,Wei-Hong JIANG,Guo-Ping ZHAO.Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32[J].Acta Biochimica et Biophysica Sinica,2006,38(12):831-843. 被引量：6
  • 3Jin Z H, Lei Y L, Lin J P, et al, Improvement of pristinamycinproducing Streptomyces pristinaespiralis by rational screening [ J ]. World J Microbiol Biotechnol,2005, 22 (2) : 129-134.
  • 4Folcher M, Gaillard H, Nguyen L T, et al. Pleiotropic functions of a Streptomyces prlstlnaespiralis autoregulator receptor in development, antibiotic biosynthesis, and expression of a superoxide dismutase [ J]. J Biol Chem, 2001,276 (47) : 44297-44306.
  • 5Bamas-Jacgues N, Lorenzon S, Lacroix P, et al. Cluster organization of the genes of Streptomyces pristinaespiralis involved in pristinamycin biosynthesis and resistance elucidated by pulsed-field gel electrophoresis[J]. J Appl Microbiol, 1999,87(6):939-94-8.
  • 6Blanc V, Lagneaux D, Didier P, et al. Cloning and analysis of structural genes from Streptomyces pristinaespiralis encoding enzymes involved in the conversion of PIIB into PIIA: PIIA synthase and NADH : FMN oxidoreductase [ J ]. J Bacteriol, 1995,177 (18) : 5206- 5214.
  • 7Blanc V, Salah-Bey K, Folcher M, et al. Molecular characterization and transcriptional analysis of a muhidrug resistance gene cloned from the pristinamycin producing organism, Streptomyces pristinaespiralis [J]. Mol Microbiol, 1995,17(5) :989-999.
  • 8Bentley S D, Chater K F, Cerdeno-Tarraga A M, et al. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) [J]. Nature,2002,417(6885) : 141-147.
  • 9Lee P C, Umeyama T, Hofinouchi S. AfsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3 ( 2 ) [J].Mol Microbiol, 2002,43(6) : 1413-1430.
  • 10Xu B, Jin Z H, Wang H Z, et al. Evolution of Streptomyces pdstinaesplralis for resistance and production of pristinamyein by genome shuffling[J] .Appl Mierobiol Biotechnol, 2008, 80(2):261- 267.

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中国生物化学与分子生物学报
2008年 第6期

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