华美游仆虫细胞微管胞器的直接荧光和免疫荧光标记

Microtubular Organelles in Euplotes elegans Revealed by Direct-fluorescent and Immunofluorescent Labeling

应用直接荧光和免疫荧光标记显示,腹毛目纤毛虫华美游仆虫(Euplotes elegans)细胞微管胞器由口围带、波动膜、额腹横棘毛、缘棘毛、尾棘毛、背触毛等纤毛器微管以及纤毛器基部附属微管和非纤毛区皮层微管骨架组成。其中,口围带基部含有小膜托架、小膜附属微管,波动膜基部含有波动膜托架,额腹横棘毛基部含有前纵微管束、后纵微管束、横微管束或放射微管束,左缘棘毛和尾棘毛基部微管束分化不明显,背纤毛基部含有玫瑰花状的基体周围骨架,这些微管结构与细胞背腹面皮层纵微管与横微管网一起组织成该类纤毛虫的主要皮层细胞骨架。结果表明,游仆虫皮层细胞骨架是以微管为主要成分构建而成的,并且其棘毛基部微管的组成具有与其他类纤毛虫不同的特征;游仆虫间期细胞及形态发生时期纤毛基体或纤毛原基中存在中心蛋白,其可能与纤毛基体结构的维持及基体发生过程中微管的组装有关。

The microtubular organelles in the hypotrich ciliate Euplotes elegans were analyzed with fluorescence labeling of FLUTAX, anti-α tubulin and anti-centrin antibody. The results showed that these organelles consist of adoral zone of membranelles （ AZM ）, undulating membranes （ UM ）, frontal-ventral-transverse cirri （ FVTC ）, marginal cirri （MC）, caudal cirri （CC）, dorsal kineties （DK） and the base associated microtubules of these ciliatures. The microtubular cytoskeleton of AZM comprises membranelle brackets and its associated microtubules ; the base associated microtubules of FVTC contained anterior longitudinal microtubules （ALM）, posterior longitudinal microtubules （PLM）, transverse microtubules （TM） or radiating microtubules （RM）. The base associated microtubules of the left MC and CC differentiated less obviously, and the basal bodies of DK contained rosette-like skeletal structure. All these microtubular structures together with the longitudinal microtubules and the transverse microtubules of dorsoventral cortex make up the cytoskeleton. Meanwhile, our results also indicate that the cytoskeleton of Euplotes is built up mainly with the microtubules as its major units, and the organization of its base-associated microtubules is quite different from other ciliates. Moreover, it was found that centrin existed in the anlagen and the basal bodies during the process of the morphogenesis, which might play a role in the maintenance of basal bodies and the assembly of microtubules.