二种快速ELISA用于检测囊虫病患者血清特异性抗体的实验观察

Experimental Observations of Serum Specific Antiboday on Patients with Cysticercosis by two quick ELISA

用快速ＩｇＧ－ＥＬＩＳＡ和快速ＳＰＡ－ＥＬＩＳＡ检测囊虫病人血清抗体，其阳性率分别为９０．００％和９４．２９％，两虫之间无显著性差异（Ｐ＞０．０５）。两法假阴性率分别为１．４３％和２．８６％，对包虫病人血清出现较强交叉反应，对肝吸虫、肺吸虫病人血清出现一定程度的交叉反应，而对血吸虫病人、绦虫病人血清未出现交叉反应。若两种方法同时使用可提高敏感性。两种方法均较简便，在包虫病非流行区可作为较好的流行病学调查筛检方法。

Serum antibody on patients with cysticercosis was estimated by quick IgG- ELISA and quick SPA-ELISA.The positivities of the quick IgG-ELISA and the quick spa-ELISA were 90.00% and 94.29 respectively. There was no difference between them(P>0.05).The false-positivities of the two methods were 1.43% and 2.86%.The methods can cross-react with the serum of hydatidosis patients strongly and of clonorchiasis and paragonimiasis patients to some extent.There was no cross-reaction with the serum of the schistosomiasis patients.The sensitivity could be enhanced if the two methods are used at the same time.The results suggested that the quick IgG-ELISA and the quick SPA-ELISA are simple and could be used as better mass-sereening methods of epidemiological investigation as well as chinicall in non-epidemic areas of hydatidosis.