缺血再灌注对小鼠学习记忆的损伤及山茶花提取物的保护作用

The protective effects of the extract of Camellia japonica L.on learning-memory dysfunction induced by ischemia-reperfusion in mice and its relationship with inflammation and blood-brain barrier damage

采用山茶花提取物探讨对缺血性记忆障碍的保护作用及其与炎症和血脑屏障损伤的关系的试验研究。其方法为给药后第7天,采用双侧颈总动脉结扎法建立重复前脑缺血再灌注模型。术后第7天用水迷宫检测小鼠学习记忆能力;用紫外分光光度计检测脑组织髓过氧化物酶(MPO)活性和伊文思蓝(EB)含量;同时测定脑组织的含水量。前脑重复缺血再灌注使小鼠的学习记忆能力显著下降(P<0.01),同时伴随脑内MPO活性上升(P<0.01),脑组织EB及水分量升高(P<0.01)。山茶花提取物预防和治疗给药可提高缺血小鼠的学习记忆能力;并使脑组织EB含量下降(P<0.05和P<0.01),脑水分含量减少(P<0.05),血脑屏障损伤程度有所减轻;同时伴随MPO活性下降(P<0.05和P<0.01),炎症反应减弱。山茶花提取物能减轻缺血再灌注引起的脑损伤,降低小鼠学习记忆能力,该作用可能与其提高脑组织抗血脑屏障损伤和抗炎症反应能力有关。

To study the protective effects of the extract of Camellia japonica L. on learning-memory dysfunction induced by ischemia-raperfusion in mice and its relationship with inflammation and blood-brain barrier damage, on day 7 after drug treatment, repeated cerebral ischemiareperfusion was induced by fastening bilateral common carotid artery. The 7th day following surgery,learning-memory was measured using Morris water maze. The activity of MPO and the content of EB were then measured by Uv-photospectrometer analysis system. Meanwhile,the content of water were detected. The results showed that,ischemia-reperfusion impaired learning-memory （P 〈 0.05 and P 〈 0.01 ）, and increased the activity of MPO and the content of EB and water in mice （ P 〈 0.01 ）. It was found that the extract of Camellia japonica L. can make blood-brain barrier less serious and ameliorate neutrophil infiltration, remarkably decreasing the content of EB （P 〈 0.01 ） ,water （P 〈 0.05 ）, and the activity of MPO （ P 〈 0.05 and P 〈 0.01 ） when compared to the model group, finally dramatically improve the ability of learning-memory after ischemia-reperfusion （ P 〈 0.05, P 〈 0.01 ）. The extract of Camellia japonica L. showed an improvement effect on the memory impairment in mice induced by ischemia/reperfusion. Improvement of the anti-blood-brain-barrier-damage and anti-inflammation in brain may be involved in.