查尔酮异构酶基因的克隆序列分析及在大肠杆菌中的表达

MOLECULAR CLONING, SEQUENCING OF CHALCONE ISOMERASE GENE AND ITS EXPRESSION IN E. COLI

从矮牵牛(Petunia hybrida Vilm.)花瓣的cDNA中克隆了查尔酮异构酶(chalcone isomerase,CHI)的基因chi-a,进行了序列分析。结果表明,chi-a基因全长726 bp,编码241个氨基酸。并在大肠杆菌中表达了chi-a基因,对来源于不同植物种的CHI进行了同源性比较分析。

Anthocyanidins belong to a class of biosynthetic metabolites of flavonoids in higher plants. Chalcone isomerase (CHI) catalyzes the conversion of naringenin chalcone into flavanone. Chi-a gene was cloned from Petunia hybrida Vilm. The sequence of the coding region was 726 bp, encoding a protein of 241 amino acids and was expressed in E. coli. The amino acid sequence ho-mology was compared with CHI from a variety with plant species.